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Product Info Summary
| SKU: | M01124 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, IHC |
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Product info
Product Name
Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) Monoclonal Antibody
View all Podoplanin Antibodies
SKU/Catalog Number
M01124
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) Monoclonal Antibody (Catalog # M01124). Tested in ELISA, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01124)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: PDPN/1433
Isotype
IgG1, kappa
Immunogen
Recombinant human Podoplanin (PDPN) protein fragment (around aa 24-126) (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01124 is reactive to PDPN in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
16698 MW
Background of Podoplanin
This monoclonal antibody recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas,Kaposi sarcomas,and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01124 is guaranteed for ELISA, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (For coating, order antibody without BSA)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Cervix stained with Anti-Podoplanin-Monospecific Mouse Monoclonal Antibody (PDPN/1433)
Protein Target Info & Infographic
Gene/Protein Information For PDPN (Source: Uniprot.org, NCBI)
Gene Name
PDPN
Full Name
Podoplanin
Weight
16698 MW
Superfamily
podoplanin family
Alternative Names
Podoplanin;Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A;PDPN ;GP36 ;PSEC0003, PSEC0025; PDPN AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, T1A2, TI1A podoplanin podoplanin|PA2.26 |T1-alpha|glycoprotein 36|glycoprotein, 36-KD|hT1alpha-1|hT1alpha-2|lung type I cell membrane associated glycoprotein|lung type-I cell membrane-associated glycoprotein (T1A-2)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDPN, check out the PDPN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDPN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) Monoclonal Antibody (M01124)
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