Anti-Podoplanin/gp36/PDPN Antibody Picoband®

Product Name

Anti-Podoplanin/gp36/PDPN Antibody Picoband®

View all Podoplanin Antibodies

SKU/Catalog Number

PA1675

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Podoplanin/gp36/PDPN Antibody catalog # PA1675. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Podoplanin/gp36/PDPN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1675)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat Podoplanin/gp36, different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1675 is reactive to Pdpn in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

38 kDa

Calculated molecular weight

17579 MW

Background of Podoplanin

PDPN (Podoplanin), also called T1A, T1A2, GP36, OTS8 or AGGRUS, is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The PDPN gene is mapped to chromosome 1 by the International radiation Hybrid mapping consortium. The physiological function of PDPN may be related to its mucin-type character. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Immunohistochemical analysis of PDPN in placenta, kidney, lung, and nasal polyps showed expression at the apical plasma membrane of vascular endothelial cells and in alveolar epithelial cells. Overexpression of rat PDPN in human and rodent endothelial cells promoted formation of elongated cell extensions and significantly increased endothelial cell adhesion, migration, and tube formation. Inhibition of PDPN expression by small interfering RNAs decreased cell adhesion in cultured human dermal lymphatic endothelial cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1675 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Positive Control

WB: rat lung tissue, mouse lung tissue

Validation Images & Assay Conditions

Gene/Protein Information For Pdpn (Source: Uniprot.org, NCBI)

Alternative Names

Podoplanin;E11 antigen epitope;Glycoprotein 38;Gp38;OTS-8;RTI140;T1-alpha;T1A;Type I cell 40 kDa protein;Pdpn;Gp38, Ots8; PDPN AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, T1A2, TI1A podoplanin podoplanin|PA2.26 |T1-alpha|glycoprotein 36|glycoprotein, 36-KD|hT1alpha-1|hT1alpha-2|lung type I cell membrane associated glycoprotein|lung type-I cell membrane-associated glycoprotein (T1A-2)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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