Product Info Summary
SKU: | A05460 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PNPase PNPT1 Antibody
SKU/Catalog Number
A05460
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PNPase PNPT1 Antibody catalog # A05460. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PNPase PNPT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05460)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PNPase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05460 is reactive to Pnpt1 in Human, Mouse
Applications
A05460 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
85951 MW
Background of Pnpt1
Isoform GANP:Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells By similarity. Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Isoform MCM3AP:Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.
Nobuo Sakaguchi, J. Immunol., Apr 2005; 174: 4485 - 4494.
Kazuhiko Kuwahara, Blood, Apr 2000; 95: 2321 - 2328.
Y Kono, K Maeda, Genes Cells, Aug 2002; 7: 821 - 834.
Kazuhiko Kuwahara, PNAS, Aug 2001; 98: 10279.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-PNPase PNPT1 Antibody A05460
Western Blot (WB) analysis of specific cells using PNPase polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For Pnpt1 (Source: Uniprot.org, NCBI)
Gene Name
Pnpt1
Full Name
Polyribonucleotide nucleotidyltransferase 1, mitochondrial
Weight
85951 MW
Superfamily
polyribonucleotide nucleotidyltransferase family
Alternative Names
DKFZp762K1914; EC 2.7.7; EC 2.7.7.8; mitochondrial; OLD35; old-35; PNPase 1; PNPase old-35; PNPase; Polynucleotide phosphorylase 1; Polynucleotide phosphorylase-like protein; polyribonucleotide nucleotidyltransferase 1 Pnpt1|1200003F12Rik, Old35, P, PNPase, Pnptl1|polyribonucleotide nucleotidyltransferase 1|polyribonucleotide nucleotidyltransferase 1, mitochondrial|3-5 RNA exonuclease OLD35|PNPase 1|PNPase old-35|polynucleotide phosphorylase 1|polynucleotide phosphorylase-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pnpt1, check out the Pnpt1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pnpt1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PNPase PNPT1 Antibody (A05460)
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