Anti-CstF-64 (R46) CSTF2 Antibody

Product Name

Anti-CstF-64 (R46) CSTF2 Antibody

View all Cleavage stimulation factor 2 Antibodies

SKU/Catalog Number

A07037

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CstF-64 (R46) CSTF2 Antibody catalog # A07037. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CstF-64 (R46) CSTF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07037)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human SAR1B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07037 is reactive to CSTF2 in Human, Mouse, Rat

Applications

A07037 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

60959 MW

Background of Cleavage stimulation factor 2

Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.

Song H., Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
Zhou Y., Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of CSTF2 using Anti-CstF-64 (R46) CSTF2 Antibody (A07037).

Immunohistochemistry (IHC) analyzes of CstF-64 (R46) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody

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Figure 1. Western blotting validation for Anti-CstF-64 (R46) CSTF2 Antibody A07037

Western blot (WB) analysis of CstF-64 (R46) pAb at 1:1000 dilution
Lane1:K562 whole cell lysate(20ug)
Lane2:HEK293T whole cell lysate(20ug)
Lane3:C6 whole cell lysate(40ug)
Lane4:SP2/0 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For CSTF2 (Source: Uniprot.org, NCBI)

Alternative Names

betaCstF-64 variant 2; CF-1 64 kDa subunit; Cleavage stimulation factor 64 kDa subunit; cleavage stimulation factor subunit 2; cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD; cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa; CSTF 64 kDa subunit; CstF-64 CSTF2 CstF-64 cleavage stimulation factor subunit 2 cleavage stimulation factor subunit 2|CF-1 64 kDa subunit|CSTF 64 kDa subunit|betaCstF-64 variant 2|cleavage stimulation factor 64 kDa subunit|cleavage stimulation factor, 3 pre-RNA, subunit 2, 64kD|cleavage stimulation factor, 3 pre-RNA, subunit 2, 64kDa

For more info on CSTF2, check out the CSTF2 Infographic

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In this infographic, you will see the following information for CSTF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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