Anti-PNAS4 DESI2 Antibody

Product Name

Anti-PNAS4 DESI2 Antibody

View all PPPDE1 Antibodies

SKU/Catalog Number

A14424

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PNAS4 DESI2 Antibody (Catalog # A14424). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PNAS4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PNAS4 DESI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14424)

Host

Rabbit

Contents

PNAS4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PNAS4 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PNAS4. The immunogen is located within the last 50 amino acids of PNAS4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

SIGLEC15 antibody is predicted to not cross-react with other SIGLEC family members.

Reactive Species

A14424 is reactive to DESI2 in Human, Mouse, Rat

Applications

A14424 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

21444 MW

Background of PPPDE1

PNAS4 is a highly conserved protein that shares high homology from plants to animals. It was initially identified as a putative apoptosis-related protein in the human acute promyelocytic leukemia cell line NB4. PNAS4 is activated during the early response to DNA damage and can inhibit proliferation via apoptosis when overexpressed in some tumor cells such as U2OS, SKOV3, and A549. PNAS4 inhibits tumor cell proliferation through the following mechanisms: (i) overexpression of PNAS4 causes S phase arrest by regulating the expression of cell cycle-related proteins and (ii) PNAS4 induces apoptosis through the mitochondrial apoptosis pathway. Recent evidence has shown that PNAS4 may be involved in the genesis of some cancers and could be a potential candidate for lung cancer therapy alone or in combination with gemcitabine. At least two isoforms of PNAS4 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PNAS4 antibody can be used for detection of PNAS4 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of PNAS4 in EL4 cell lysate with PNAS4 antibody at (A) 1 and (B) 2 μg/mL.

Gene/Protein Information For DESI2 (Source: Uniprot.org, NCBI)

Alternative Names

C1orf121; CGI-146; chromosome 1 open reading frame 121; FAM152A; FLJ21998; member A; PNAS-4; PPPDE peptidase domain containing 1; PPPDE peptidase domain-containing protein 1; Protein FAM152A DESI2 C1orf121, CGI-146, DESI, DESI1, DeSI-2, FAM152A, PNAS-4, PPPDE1 desumoylating isopeptidase 2 deubiquitinase DESI2|PPPDE peptidase domain-containing protein 1|desumoylating isopeptidase 1|family with sequence similarity 152, member A

For more info on DESI2, check out the DESI2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DESI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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