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Product Info Summary
| SKU: | M00093 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PML Rabbit Monoclonal Antibody
View all PML Protein Antibodies
SKU/Catalog Number
M00093
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PML Rabbit Monoclonal Antibody catalog # M00093. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PML Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00093)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABAF-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PML
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00093 is reactive to PML in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
97551 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00093 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
IP 1:50
FC 1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of PML expression in 293 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PML (Source: Uniprot.org, NCBI)
Gene Name
PML
Full Name
Protein PML
Weight
97551 MW
Alternative Names
Protein PML;Promyelocytic leukemia protein;RING finger protein 71;Tripartite motif-containing protein 19;PML;MYL, PP8675, RNF71, TRIM19; PML MYL, PP8675, RNF71, TRIM19 PML nuclear body scaffold protein PML|PML/RARA fusion|RING finger protein 71|probable transcription factor PML|promyelocytic leukemia protein|promyelocytic leukemia, inducer of|tripartite motif protein TRIM19|tripartite motif-containing protein 19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PML, check out the PML Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PML: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PML Rabbit Monoclonal Antibody (M00093)
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No publications found for M00093
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Customer Q&As
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16 Customer Q&As for Anti-PML Rabbit Monoclonal Antibody
Question
Will anti-PML Rabbit Monoclonal antibody M00093 work on feline IP with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Our lab technicians have not tested anti-PML Rabbit Monoclonal antibody M00093 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PML Rabbit Monoclonal antibody M00093 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cervix carcinoma erythroleukemia in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-06
Question
Is a blocking peptide available for product anti-PML Rabbit Monoclonal antibody (M00093)?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
We do provide the blocking peptide for product anti-PML Rabbit Monoclonal antibody (M00093). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-08
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-PML Rabbit Monoclonal antibody M00093. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-31
Question
We have observed staining in rat cervix carcinoma. Are there any suggestions? Is anti-PML Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
From literature cervix carcinoma does express PML. From Uniprot.org, PML is expressed in left lobe of thyroid gland, brain, kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PML expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 16572171
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-02-05
Question
Would M00093 anti-PML Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
As indicated on the product datasheet, M00093 anti-PML Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-09
Question
I have attached the WB image, lot number and protocol we used for kidney using anti-PML Rabbit Monoclonal antibody M00093. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-28
Question
We are currently using anti-PML Rabbit Monoclonal antibody M00093 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The anti-PML Rabbit Monoclonal antibody (M00093) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-31
Question
Do you have a BSA free version of anti-PML Rabbit Monoclonal antibody M00093 available?
Verified Customer
Verified customer
Asked: 2019-04-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PML Rabbit Monoclonal antibody M00093 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-23
Question
Would anti-PML Rabbit Monoclonal antibody M00093 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
According to the expression profile of kidney, PML is highly expressed in kidney. So, it is likely that anti-PML Rabbit Monoclonal antibody M00093 will work for WB with kidney.
Boster Scientific Support
Answered: 2019-03-15
Question
I see that the anti-PML Rabbit Monoclonal antibody M00093 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-26
Question
We ordered your anti-PML Rabbit Monoclonal antibody for WB on cervix carcinoma erythroleukemia in the past. I am using rat, and We intend to use the antibody for IF next. I am interested in examining cervix carcinoma erythroleukemia as well as kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
I took a look at the website and datasheets of our anti-PML Rabbit Monoclonal antibody and I see that M00093 has been validated on rat in both WB and IF. Thus M00093 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-11-30
Question
My colleagues were content with the WB result of your anti-PML Rabbit Monoclonal antibody. However we have been able to see positive staining in kidney nucleus. nucleus using this antibody. Is that expected? Could you tell me where is PML supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
Based on literature, kidney does express PML. Generally PML expresses in nucleus. nucleus, nucleoplasm. cytoplasm. Regarding which tissues have PML expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 16572171
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-10-25
Question
Is this M00093 anti-PML Rabbit Monoclonal antibody reactive to the isotypes of PML?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
The immunogen of M00093 anti-PML Rabbit Monoclonal antibody is A synthesized peptide derived from human PML. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-01
Question
Can you help my question with product M00093, anti-PML Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00093 anti-PML Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-16
Question
you antibody to test anti-PML Rabbit Monoclonal antibody M00093 on human kidney for research purposes, then I may be interested in using anti-PML Rabbit Monoclonal antibody M00093 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
The products we sell, including anti-PML Rabbit Monoclonal antibody M00093, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-15
Question
I was wanting to use your anti-PML Rabbit Monoclonal antibody for WB for human kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney identification?
O. Wu
Verified customer
Asked: 2015-05-07
Answer
As indicated on the product datasheet, M00093 anti-PML Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-05-07
