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Product Info Summary
| SKU: | A03162-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Plunc/BPIFA1 Antibody Picoband®
SKU/Catalog Number
A03162-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Plunc/BPIFA1 Antibody Picoband® catalog # A03162-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Plunc/BPIFA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03162-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Plunc/BPIFA1 recombinant protein (Position: L64-V256).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03162-2 is reactive to BPIFA1 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
27 kDa
Calculated molecular weight
65411 MW
Background of PLUNC
Palate, lung, and nasal epithelium clone protein[5] (PLUNC) is a gene encoding a secretory protein. This gene is the human homolog of murine plunc, and like the mouse gene, is specifically expressed in the upper airways and nasopharyngeal regions. The encoded antimicrobial protein displays antibacterial activity against Gram-negative bacteria. It is thought to be involved in inflammatory responses to irritants in the upper airways and may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Multiple transcript variants resulting from alternative splicing in the 3' UTR have been detected, but the full-length nature of only three are known.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03162-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Plunc/BPIFA1 using anti-Plunc/BPIFA1 antibody (A03162-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plunc/BPIFA1 antigen affinity purified polyclonal antibody (Catalog # A03162-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plunc/BPIFA1 at approximately 27 kDa. The expected band size for Plunc/BPIFA1 is at 27 kDa.
Protein Target Info & Infographic
Gene/Protein Information For BPIFA1 (Source: Uniprot.org, NCBI)
Gene Name
BPIFA1
Full Name
BPI fold-containing family A member 1
Weight
65411 MW
Superfamily
BPI/LBP/Plunc superfamily
Alternative Names
IFNGR2 protein; Interferon gamma receptor 2; IFNGR2; mCG_11456 Bpifa1|LUNX, NASG, Pl, Plunc, SPL, SPLUNC1, SPURT|BPI fold containing family A, member 1|BPI fold-containing family A member 1|palate lung and nasal epithelium clone protein|palate, lung, and nasal epithelium associated|palate, lung, and nasal epithelium clone|palate, lung, and nasal epithelium expressed transcript|protein Plunc
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BPIFA1, check out the BPIFA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BPIFA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Plunc/BPIFA1 Antibody Picoband® (A03162-2)
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