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Product Info Summary
| SKU: | PA1309 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospholamban/PLN Antibody Picoband®
View all Phospholamban Antibodies
SKU/Catalog Number
PA1309
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Phospholamban/PLN Antibody catalog # PA1309. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospholamban/PLN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1309)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PLN, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1309 is reactive to PLN in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
24 kDa, 12 kDa
Calculated molecular weight
6109 MW
Background of Phospholamban
Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2+ pump in cardiac muscle and skeletal muscle cells. The subsequent activation of the Ca (2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Phospholamban is also expressed in slow-twitch skeletal muscle and some smooth muscle cells. McTiernan et al. (1999) observed that human ventricle and quadriceps displayed high levels of phospholamban transcripts and proteins, with markedly lower expression observed in smooth muscles, while the right atrium also expressed low levels of phospholamban. The structure of the human phospholamban gene closely resembles that reported for chicken, rabbit, rat, and mouse. Comparison of the human to other mammalian phospholamban genes indicated a marked conservation of sequence for at least 217 bp upstream of the transcription start site.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1309 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat heart tissue, rat heart tissue, mouse heart tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PLN using anti-PLN antibody (PA1309).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLN antigen affinity purified polyclonal antibody (Catalog # PA1309) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected respectively for PLN at approximately 24 kDa and 12 kDa. The expected band size for PLN is at 6 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PLN (Source: Uniprot.org, NCBI)
Gene Name
PLN
Full Name
Cardiac phospholamban
Weight
6109 MW
Superfamily
phospholamban family
Alternative Names
Cardiac phospholamban;PLB;PLN;PLB; PLN CMD1P, CMH18, PLB phospholamban cardiac phospholamban
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLN, check out the PLN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospholamban/PLN Antibody Picoband® (PA1309)
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No publications found for PA1309
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Customer Q&As
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5 Customer Q&As for Anti-Phospholamban/PLN Antibody Picoband®
Question
I am looking for to test anti-Phospholamban/PLN antibody PA1309 on human liver for research purposes, then I may be interested in using anti-Phospholamban/PLN antibody PA1309 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
The products we sell, including anti-Phospholamban/PLN antibody PA1309, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-06
Question
Will PA1309 anti-Phospholamban/PLN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
You can see on the product datasheet, PA1309 anti-Phospholamban/PLN antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-28
Question
Would anti-Phospholamban/PLN antibody PA1309 work on primate WB with liver?
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
Our lab technicians have not validated anti-Phospholamban/PLN antibody PA1309 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Phospholamban/PLN antibody PA1309 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-11
Question
Will anti-Phospholamban/PLN antibody PA1309 work for WB with liver?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
According to the expression profile of liver, PLN is highly expressed in liver. So, it is likely that anti-Phospholamban/PLN antibody PA1309 will work for WB with liver.
Boster Scientific Support
Answered: 2018-05-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Phospholamban/PLN antibody PA1309. Let me know if you need anything else.
A. Singh
Verified customer
Asked: 2016-03-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-29
