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Product Info Summary
| SKU: | A12553 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-PLA2G4D Antibody
SKU/Catalog Number
A12553
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PLA2G4D Antibody (Catalog# A12553). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLA2G4D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12553)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PLA2G4D. Immunogen sequence location: 531-580
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12553 is reactive to PLA2G4D in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
91952 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12553 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain, using PLA2G4D Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For PLA2G4D (Source: Uniprot.org, NCBI)
Gene Name
PLA2G4D
Full Name
Cytosolic phospholipase A2 delta
Weight
91952 MW
Alternative Names
PLA2G4D;Cytosolic phospholipase A2 delta;cPLA2-delta;Phospholipase A2 group IVD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLA2G4D, check out the PLA2G4D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLA2G4D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLA2G4D Antibody (A12553)
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No publications found for A12553
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Customer Q&As
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3 Customer Q&As for Anti-PLA2G4D Antibody
Question
We are currently using anti-PLA2G4D antibody A12553 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
C. Anderson
Verified customer
Asked: 2020-01-23
Answer
The anti-PLA2G4D antibody (A12553) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-23
Question
Is there a BSA free version of anti-PLA2G4D antibody A12553 available?
J. Anderson
Verified customer
Asked: 2017-10-11
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PLA2G4D antibody A12553 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-11
Question
Can you help my question with product A12553, anti-PLA2G4D antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Huang
Verified customer
Asked: 2014-11-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12553 anti-PLA2G4D antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-11-12
