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Product Info Summary
| SKU: | M02259-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PLA2G2A Rabbit Monoclonal Antibody
SKU/Catalog Number
M02259-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-PLA2G2A Rabbit Monoclonal Antibody catalog # M02259-1. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLA2G2A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02259-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
25P80
Isotype
IgG
Immunogen
A synthesized peptide derived from human PLA2G2A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02259-1 is reactive to PLA2G2A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
16 kDa
Calculated molecular weight
32385 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02259-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: human A549 whole cell, human HepG2 whole cell, human RT4 whole cell, human Hacat whole cell, rat small intestine tissue, rat C6 whole cell, mouse small intestine tissue, mouse EL-4 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PLA2G2A using anti-PLA2G2A antibody (M02259-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat small intestine tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse small intestine tissue lysates,
Lane 8: mouse EL-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G2A antigen affinity purified monoclonal antibody (Catalog # M02259-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2G2A at approximately 16 kDa. The expected band size for PLA2G2A is at 16 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PLA2G2A (Source: Uniprot.org, NCBI)
Gene Name
PLA2G2A
Full Name
Phospholipase A2, membrane associated
Weight
32385 MW
Superfamily
phospholipase A2 family
Alternative Names
Oligodendrocyte transcription factor 2;Oligo2;Class B basic helix-loop-helix protein 1;bHLHb1;Class E basic helix-loop-helix protein 19;bHLHe19;Protein kinase C-binding protein 2;Protein kinase C-binding protein RACK17;OLIG2;BHLHB1, BHLHE19, PRKCBP2, RACK17; PLA2G2A MOM1, PLA2, PLA2B, PLA2L, PLA2S, PLAS1, sPLA2 phospholipase A2 group IIA phospholipase A2, membrane associated|GIIC sPLA2|NPS-PLA2|group IIA phospholipase A2|non-pancreatic secretory phospholipase A2|phosphatidylcholine 2-acylhydrolase 2A|phospholipase A2, group IIA (platelets, synovial fluid)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLA2G2A, check out the PLA2G2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLA2G2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLA2G2A Rabbit Monoclonal Antibody (M02259-1)
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