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Product Info Summary
| SKU: | A01796 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PKCz(Ab-410) PRKCZ Antibody
SKU/Catalog Number
A01796
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PKCz(Ab-410) PRKCZ Antibody (Catalog # A01796). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKCz(Ab-410) PRKCZ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01796)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 408~412 ( T-S-T-F-C) derived from PKCz
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01796 is reactive to PRKCZ in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
67660 MW
Background of PKC zeta
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
Parmentier JH, et al. (2004) BMC Cell Biol; 5: 4.
Castrillo A, et al. (2003) Mol Cell Biol; 23(4): 1196-1208.
Paramio JM, et al. ( 2001) Mol Cell Biol; 21(21): 7449-7459.
Braiman L, et al. (2001) Mol Cell Biol; 21(22): 7852-7861.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01796 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 78kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extract from 293 cells and Hela using PKCz(Ab-410) antibody A01796.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCz(Ab-410) antibody(A01796).
Protein Target Info & Infographic
Gene/Protein Information For PRKCZ (Source: Uniprot.org, NCBI)
Gene Name
PRKCZ
Full Name
Protein kinase C zeta type
Weight
67660 MW
Superfamily
protein kinase superfamily
Alternative Names
Protein kinase C zeta type;2.7.11.13;nPKC-zeta;PRKCZ;PKC2; PRKCZ PKC-ZETA, PKC2 protein kinase C zeta protein kinase C zeta type|nPKC-zeta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCZ, check out the PRKCZ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCZ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKCz(Ab-410) PRKCZ Antibody (A01796)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-PKCz(Ab-410) PRKCZ Antibody
Question
Would anti-PKCz(Ab-410) antibody A01796 work on canine WB with neuroblastoma?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Our lab technicians have not validated anti-PKCz(Ab-410) antibody A01796 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PKCz(Ab-410) antibody A01796 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine neuroblastoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-23
Question
I would like to test anti-PKCz(Ab-410) antibody A01796 on mouse liver for research purposes, then I may be interested in using anti-PKCz(Ab-410) antibody A01796 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The products we sell, including anti-PKCz(Ab-410) antibody A01796, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-29
Question
Is a blocking peptide available for product anti-PKCz(Ab-410) antibody (A01796)?
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
We do provide the blocking peptide for product anti-PKCz(Ab-410) antibody (A01796). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-17
Question
Is this A01796 anti-PKCz(Ab-410) antibody reactive to the isotypes of PRKCZ?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
The immunogen of A01796 anti-PKCz(Ab-410) antibody is Peptide sequence around aa. 408~412 ( T-S-T-F-C) derived from PKCz . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-14
