Click image to see more details
Product Info Summary
| SKU: | M00822 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PKC delta PRKCD Rabbit Monoclonal Antibody
SKU/Catalog Number
M00822
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PKC delta PRKCD Rabbit Monoclonal Antibody catalog # M00822. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC delta PRKCD Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00822)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IHD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PKC delta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00822 is reactive to PRKCD in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
78 kDa
Calculated molecular weight
77505 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00822 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRKCD using anti-PRKCD antibody (M00822).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCD antigen affinity purified polyclonal antibody (Catalog # M00822) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCD at approximately 78 kDa. The expected band size for PRKCD is at 78 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PRKCD (Source: Uniprot.org, NCBI)
Gene Name
PRKCD
Full Name
Protein kinase C delta type
Weight
77505 MW
Superfamily
protein kinase superfamily
Alternative Names
Protein kinase C delta type;2.7.11.13;Tyrosine-protein kinase PRKCD;2.7.10.2;nPKC-delta;Protein kinase C delta type regulatory subunit;Protein kinase C delta type catalytic subunit;Sphingosine-dependent protein kinase-1;SDK1;PRKCD; PRKCD ALPS3, CVID9, MAY1, PKCD, nPKC-delta protein kinase C delta protein kinase C delta type|protein kinase C delta VIII|tyrosine-protein kinase PRKCD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCD, check out the PRKCD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKC delta PRKCD Rabbit Monoclonal Antibody (M00822)
Hello CJ!
M00822 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shi Y, Jiang R, Qin X, Gao A, Hou X, Chen L, Xu X, Guo Y, Chai L, Zhao L, Du X, Wu F. Up-regulation of nPKC contributes to proliferation of mice pulmonary artery smooth muscle cells in hypoxia-induced pulmonary hypertension. Eur J Pharmacol. 2021 Mar 18:174046. doi: 10.1016/j.ejphar.2021.174046. Epub ahead of print. PMID: 33745958.
Species: Mouse
M00822 usage in article: APP:WB, SAMPLE:PASMCS, DILUTION:1:5000
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