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Product Info Summary
| SKU: | RP1058 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-PKC delta/PRKCD Antibody Picoband®
SKU/Catalog Number
RP1058
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PKC delta/PRKCD Antibody catalog # RP1058. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC delta/PRKCD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1058)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PKC delta recombinant protein (Position: M1-E160). Human PKC delta shares 95% and 93% amino acid (aa) sequence identity with mouse and rat PKC delta, respctively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1058 is reactive to PRKCD in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
77 kDa
Calculated molecular weight
77505 MW
Background of PKC delta
Protein kinase C delta type, also called PKCD, is an enzyme that in humans is encoded by the PRKCD gene. The PRKCD gene encodes a member of the protein kinase C family, members of which are critical for regulation of cell survival, proliferation, and apoptosis. This gene is mapped to 3p21.1. Studies both in human and mice demonstrate that this kinase is involved inB cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PRKCD is also regulated by phosphorylation on various tyrosine residues including Y311 (by SRC). It has been showed that PRKCD phosphorylates NLRC4 and that this phosphorylation is critical for inflammasome assembly. What’s more, it is also a part of a noncanonical WNT signaling cascade.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1058 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Positive Control
WB: HELA Whole Cell, SMMC Whole Cell, U87 Whole Cell, JURKAT Whole Cell, CEM Whole Cell, A549 Whole Cell
ICC/IF: A549 cell
Validation Images & Assay Conditions
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nti-PKC delta antibody, RP1058, Western blotting
All lanes: Anti PKC delta (RP1058) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Predicted bind size: 77KD
Observed bind size: 77KD
Click image to see more details
Figure 2. IF analysis of PKC delta using anti-PKC delta antibody (RP1058).
PKC delta was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PKC delta Antibody (RP1058) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PRKCD (Source: Uniprot.org, NCBI)
Gene Name
PRKCD
Full Name
Protein kinase C delta type
Weight
77505 MW
Superfamily
protein kinase superfamily
Alternative Names
Protein kinase C delta type;2.7.11.13;Tyrosine-protein kinase PRKCD;2.7.10.2;nPKC-delta;Protein kinase C delta type regulatory subunit;Protein kinase C delta type catalytic subunit;Sphingosine-dependent protein kinase-1;SDK1;PRKCD; PRKCD ALPS3, CVID9, MAY1, PKCD, nPKC-delta protein kinase C delta protein kinase C delta type|protein kinase C delta VIII|tyrosine-protein kinase PRKCD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCD, check out the PRKCD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKC delta/PRKCD Antibody Picoband® (RP1058)
Hello CJ!
RP1058 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors
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Customer Q&As
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3 Customer Q&As for Anti-PKC delta/PRKCD Antibody Picoband®
Question
I was wanting to use your anti-PKC delta/PRKCD antibody for WB for human platelet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human platelet identification?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
It shows on the product datasheet, RP1058 anti-PKC delta/PRKCD antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-13
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-PKC delta/PRKCD antibody RP1058. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-17
Question
We are currently using anti-PKC delta/PRKCD antibody RP1058 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
E. Huang
Verified customer
Asked: 2015-12-14
Answer
The anti-PKC delta/PRKCD antibody (RP1058) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-14
