Click image to see more details
Product Info Summary
| SKU: | M04943 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PKA 2 beta PRKAR2B Rabbit Monoclonal Antibody
View all PKA 2 beta Antibodies
SKU/Catalog Number
M04943
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PKA 2 beta PRKAR2B Rabbit Monoclonal Antibody catalog # M04943. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKA 2 beta PRKAR2B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04943)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABBB-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PKA 2 beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04943 is reactive to PRKAR2B in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
135 kDa
Calculated molecular weight
46302 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04943 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human fetal brain
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PKA 2 beta expression in human fetal brain lysate.
Protein Target Info & Infographic
Gene/Protein Information For PRKAR2B (Source: Uniprot.org, NCBI)
Gene Name
PRKAR2B
Full Name
cAMP-dependent protein kinase type II-beta regulatory subunit
Weight
46302 MW
Superfamily
cAMP-dependent kinase regulatory chain family
Alternative Names
cAMP-dependent protein kinase type II-beta regulatory subunit;PRKAR2B; PRKAR2B PRKAR2, RII-BETA protein kinase cAMP-dependent type II regulatory subunit beta cAMP-dependent protein kinase type II-beta regulatory subunit|H_RG363E19.2|WUGSC:H_RG363E19.2|cAMP-dependent protein kinase type II-beta regulatory chain|protein kinase, cAMP-dependent, regulatory subunit type II beta|protein kinase, cAMP-dependent, regulatory, type II, beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAR2B, check out the PRKAR2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAR2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKA 2 beta PRKAR2B Rabbit Monoclonal Antibody (M04943)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PKA 2 beta PRKAR2B Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PKA 2 beta PRKAR2B Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-PKA 2 beta PRKAR2B Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-PKA 2 beta Rabbit Monoclonal antibody (M04943)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
We do provide the blocking peptide for product anti-PKA 2 beta Rabbit Monoclonal antibody (M04943). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-16
Question
We are currently using anti-PKA 2 beta Rabbit Monoclonal antibody M04943 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
The anti-PKA 2 beta Rabbit Monoclonal antibody (M04943) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-22
Question
My question regarding product M04943, anti-PKA 2 beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04943 anti-PKA 2 beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-16
Question
Do you have a BSA free version of anti-PKA 2 beta Rabbit Monoclonal antibody M04943 available?
D. Thomas
Verified customer
Asked: 2019-04-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PKA 2 beta Rabbit Monoclonal antibody M04943 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-15
Question
Will anti-PKA 2 beta Rabbit Monoclonal antibody M04943 work on feline WB with testis?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
Our lab technicians have not validated anti-PKA 2 beta Rabbit Monoclonal antibody M04943 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PKA 2 beta Rabbit Monoclonal antibody M04943 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-25
Question
Is this M04943 anti-PKA 2 beta Rabbit Monoclonal antibody reactive to the isotypes of PRKAR2B?
S. Carter
Verified customer
Asked: 2015-05-20
Answer
The immunogen of M04943 anti-PKA 2 beta Rabbit Monoclonal antibody is A synthesized peptide derived from human PKA 2 beta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-05-20
