Anti-PJA1 Antibody

Product Name

Anti-PJA1 Antibody

View all RNF70 Antibodies

SKU/Catalog Number

A11179

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PJA1 Antibody (Catalog # A11179). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PJA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-PJA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11179)

Host

Rabbit

Contents

PJA1 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1. The immunogen is located within amino acids 50 - 100 of PJA1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.

Reactive Species

A11179 is reactive to PJA1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

71002 MW

Background of RNF70

Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11179 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 – 0.5 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For PJA1 (Source: Uniprot.org, NCBI)

Alternative Names

Praja ring finger 1, E3 ubiquitin-protein ligase Praja-1, PRAJA1, RING finger protein 70, RNF70 Pja1|praja-1|praja ring finger ubiquitin ligase 1|E3 ubiquitin-protein ligase Praja-1|RING-type E3 ubiquitin transferase Praja-1|praja ring finger 1, E3 ubiquitin protein ligase|praja1, RING-H2 motif containing

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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