Anti-PIWI-L2 Antibody

Product Name

Anti-PIWI-L2 Antibody

View all PIWIL2 Antibodies

SKU/Catalog Number

A04654-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PIWI-L2 Antibody (Catalog # A04654-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

PIWI-L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PIWI-L2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04654-1)

Host

Rabbit

Contents

PIWI-L2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PIWI-L2 antibody was raised against a 17 amino acid synthetic peptide near the center of human PIWI-L2. The immunogen is located within amino acids 410 - 460 of PIWI-L2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of PIWI-L2 are known to exist; this antibody will only detect the two longest isoforms. PIWI-L2 antibody is predicted to not cross-react with other PIWI protein family members.

Reactive Species

A04654-1 is reactive to PIWIL2 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

109849 MW

Background of PIWIL2

PIWI-L2 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L2 has been implicated in multiple roles including stem cell self-renewal and is highly expressed in many breast cancers. In these tumors, PIWI-L2 silences the expression of STAT3, a regulator of Bcl-XL and cyclin D1, allowing the proliferation and survival of breast cancer stem cells. Recent studies have shown that overexpression of PIWI-like proteins are potential biomarkers for colon and other cancers.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04654-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PIWI-L2 antibody can be used for detection of PIWI-L2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For PIWIL2 (Source: Uniprot.org, NCBI)

Alternative Names

CT80, HILI, mili, PIWIL1L, Piwi-like protein 2, CT80 PIWIL2 CT80, HILI, PIWIL1L, mili piwi like RNA-mediated gene silencing 2 piwi-like protein 2|cancer/testis 80|piwi-like 2|piwil2-like protein|testicular tissue protein Li 141

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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