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Product Info Summary
| SKU: | EK0302 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. |
Product info
Product Name
Mouse Activin A ELISA Kit PicoKine®
SKU/Catalog Number
EK0302
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Activin A ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Inhba in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Activin A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0302)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: G311-S426
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0302 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0302 is reactive to Inhba in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
Application Guarantee
EK0302 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Activin A
Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0302-CAP | Anti-Mouse Inhba Pre-coated 96-well strip microplate | 1 |
| EK0302-ST | Mouse Inhba Standard | 2 vials, 10 ng/tube |
| EK0302-DA | Mouse Inhba Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.048 | 0.094 | 0.132 | 0.201 | 0.384 | 0.692 | 1.395 | 2.013 |
Data Example Images
Click image to see more details
Mouse Activin A PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Activin A, Dilution ratio of 1:1, concentration in serum and plasma is 62-422 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Activin A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 40 | 155 | 440 | 38 | 146 | 460 |
| Standard deviation | 3.04 | 9.92 | 24.64 | 2.24 | 11.53 | 25.76 |
| CV (%) | 7.6% | 6.4% | 5.6% | 5.9% | 7.9% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Activin A in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 40 | 34 | 36 | 38 | 37 | 2.23 | 6% |
| Sample 2 | 155 | 166 | 165 | 171 | 164 | 5.8 | 3.5% |
| Sample 3 | 440 | 452 | 419 | 478 | 447 | 21.32 | 4.7% |
Protein Target Info & Infographic
Gene/Protein Information For Inhba (Source: Uniprot.Org, NCBI)
Gene Name
Inhba
Full Name
Inhibin beta A chain
Weight
47392 MW
Superfamily
TGF-beta family
Alternative Names
Inhibin beta A chain;Activin beta-A chain;Inhba; INHBA EDF, FRP inhibin subunit beta A inhibin beta A chain|FSH-releasing protein|Inhibin, beta-1|activin beta-A chain|erythroid differentiation factor|erythroid differentiation protein|follicle-stimulating hormone-releasing protein|inhibin beta A subunit|inhibin, beta A (activin A, activin AB alpha polypeptide)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Inhba, check out the Inhba Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Inhba: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Activin A ELISA Kit PicoKine® (EK0302)
Hello CJ!
EK0302 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ginsenoside Rg1 improves pathological damages by activating the p21‑p53‑STK pathway in ovary and Bax‑Bcl2 in the uterus in premature ovarian insufficiency mouse models
Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells
Biological effects of autophagy in mice with sepsis‑induced acute kidney injury
Magnolol Attenuates Concanavalin A‐induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling
Follistatin Does Not Influence the Course of Escherichia coli K1 Sepsis in a Mouse Model
Inhibition by microbial metabolites of Chinese dark tea of age-related neurodegenerative disorders in senescence-accelerated mouse prone 8 (SAMP8) mice
He L,Wang X,Cheng D,Xiong Z,Liu X. Ginsenoside Rg1 improves pathological damages by activating the p21‑p53‑STK pathway in ovary and Bax‑Bcl2 in the uterus in premature ovarian insufficiency mouse models. Mol Med Rep.2021 Jan;23(1):37.doi:10.3892/mmr.2020.
Species: Mouse
EK0302 usage in article: APP:ELISA, SAMPLE:OVARY AND UTERUS TISSUE, DILUTION:NA
Adenovirus-mediated knockdown of activin A receptor type 2A attenuates immune-induced hepatic fibrosis in mice and inhibits interleukin-17-induced activation of primary hepatic stellate cells
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Mouse Activin A ELISA Kit PicoKine®
Question
What are immunized animals, clonality and the purification method of capture/detection antibody of EK0302?
Verified customer
Asked: 2022-04-20
Answer
For the Mouse Activin A ELISA Kit PicoKine® (EK0302), the capture antibody is monoclonal antibody from mouse. Purification method: Protein A or G purified from hybridoma culture supernatant. The detection antibody is monoclonal antibody from mouse. Purification method: Protein A or G purified from hybridoma culture supernatant
Boster Scientific Support
Answered: 2022-04-22
Question
Is EK0302 a recombinant protein? What is the expression system like E.Coli. CHO cells?
Verified customer
Asked: 2022-04-20
Answer
The standard in the Mouse Activin A ELISA Kit PicoKine® (EK0302) is a recombinant protein. The expression system is CHO.
Boster Scientific Support
Answered: 2022-04-22
Question
Q: how to thaw whole blood sample for Activin A ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-11-23
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Activin A for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-11-23
Question
What is the concentration of Sulfuric Acid in TMB stop solution for EK0302?
Verified customer
Asked: 2020-07-02
Answer
The concentration of Sulfuric Acid in TMB stop solution for the Mouse Activin A ELISA Kit PicoKine™ (EK0302) is 2%.
Boster Scientific Support
Answered: 2020-07-06
Question
Q: can I use citrate plasma as samples in Mouse Activin A Picokine® ELISA Kit (Catalog # EK0302)?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Activin A causing degradation of its protein structure. Activin A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Activin A ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-12-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Lopez
Verified customer
Asked: 2018-12-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-12-08
Question
Q: how to proceed with the analysis of ELISA data? I have performed Activin A level in saliva.
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-08-23
Question
Which proteins were tested to determine EK0309 has no cross-reactivity with other relevant proteins?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
We tested the following proteins for EK0309 – ALK-1, Cripto, DAN, and Osteoactivin.
Boster Scientific Support
Answered: 2018-04-05
Question
Q: What is the optimal O.D. value for Activin A ELISA kit? I performed your Activin A ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Activin A even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Activin A ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Activin A that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-02-13
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-01-30
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Activin A using the EK0302 Mouse Activin A Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-02-11
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2017-02-11
Question
Q: can you suggest the dilution ratio of serum samples for detection of Activin A in Mouse saliva? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
M. Adams
Verified customer
Asked: 2016-06-23
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Activin A ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-06-23
Question
Which protein was the antibody raised against? Keywords: dimer, monomer
Verified Customer
Verified customer
Asked: 2015-02-06
Answer
The antibody was raised against the dimer of Activin A. The dimer has two same epitopes, which enables specific binding of Activin A antibodies and minimal cross-reactivity with its family members and other sources of non-specific binding. The capture antibody and the detection antibody used in EK0302 are the same monoclonal antibodies which combine with the same two epitopes on the dimer.
Boster Scientific Support
Answered: 2015-02-06
