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Product Info Summary
| SKU: | A04533-3 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Pirh2/RCHY1 Antibody Picoband®
SKU/Catalog Number
A04533-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Pirh2/RCHY1 Antibody Picoband® catalog # A04533-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Pirh2/RCHY1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04533-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Pirh2/RCHY1 recombinant protein (Position: Q17-Q250).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04533-3 is reactive to RCHY1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
30.11kDa
Background of Pirh2
RING finger and CHY zinc finger domain-containing protein 1 is a protein that in humans is encoded by the RCHY1 gene. The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04533-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human Daudi whole cell, human 293T whole cell, rat liver tissue, rat PC-12 whole cell, mouse liver tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Pirh2/RCHY1 using anti-Pirh2/RCHY1 antibody (A04533-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pirh2/RCHY1 antigen affinity purified polyclonal antibody (Catalog # A04533-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pirh2/RCHY1 at approximately 21 kDa. The expected band size for Pirh2/RCHY1 is at 30 kDa.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-Pirh2/RCHY1 antibody (A04533-3).
Overlay histogram showing HepG2 cells stained with A04533-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Pirh2/RCHY1 Antibody (A04533-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RCHY1 (Source: Uniprot.org, NCBI)
Gene Name
RCHY1
Full Name
RING finger and CHY zinc finger domain-containing protein 1
Weight
30.11kDa
Alternative Names
Ras-related protein Rab-11B; GTP-binding protein YPT3; RAB11B; YPT3 RCHY1 ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZCHY, ZNF363 ring finger and CHY zinc finger domain containing 1 RING finger and CHY zinc finger domain-containing protein 1|CH-rich interacting match with PLAG1|E3 ubiquitin-protein ligase Pirh2|RING finger protein 199|RING-type E3 ubiquitin transferase RCHY1|androgen-receptor N-terminal-interacting protein|p53-induced protein with a RING-H2 domain|ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase|zinc finger protein 363|zinc finger, CHY-type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RCHY1, check out the RCHY1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RCHY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pirh2/RCHY1 Antibody Picoband® (A04533-3)
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