Product Info Summary
SKU: | A09313 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PION GSAP Antibody
SKU/Catalog Number
A09313
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PION GSAP Antibody (Catalog # A09313). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PION antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PION GSAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09313)
Host
Rabbit
Contents
PION Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PION antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PION. The immunogen is located within amino acids 770 - 820 of PION.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of PION are known to exist. PION antibody is predicted to not cross-react with other F-box protein family members.
Reactive Species
A09313 is reactive to GSAP in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
97802 MW
Background of GSAP
Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by one of two beta-secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99 -amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer's disease patient's brains. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and the APP C-terminal fragment, suggesting that PION may be a potential therapeutic target for the treatment of Alzheimer's disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09313 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PION antibody can be used for detection of PION by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
![a09313 anti pion gsap antibody wb testing 1 a09313 anti pion gsap antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a09313-anti-pion-gsap-antibody-wb-testing-1.jpg)
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Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
![a09313 anti pion gsap antibody ihc testing 2 a09313 anti pion gsap antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a09313-anti-pion-gsap-antibody-ihc-testing-2.jpg)
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Immunohistochemistry of PION in human brain tissue with PION antibody at 5 μg/mL.
![a09313 anti pion gsap antibody if testing 3 a09313 anti pion gsap antibody if testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a09313-anti-pion-gsap-antibody-if-testing-3.jpg)
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Immunofluorescence of PION in Human Brain cells with PION antibody at 20 μg/mL.
![a09313 anti pion gsap antibody ihc testing 4 a09313 anti pion gsap antibody ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a09313-anti-pion-gsap-antibody-ihc-testing-4.jpg)
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Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 μg/mL.
![a09313 anti pion gsap antibody if testing 5 a09313 anti pion gsap antibody if testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a09313-anti-pion-gsap-antibody-if-testing-5.jpg)
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Immunofluorescence of PION in mouse brain tissue with PION antibody at 20 μg/mL.
Red: PION Antibody (A09313)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For GSAP (Source: Uniprot.org, NCBI)
Gene Name
GSAP
Full Name
Gamma-secretase-activating protein
Weight
97802 MW
Superfamily
GSAP family
Alternative Names
Gamma-secretase-activating protein GSAP PION gamma-secretase activating protein gamma-secretase-activating protein|pigeon homolog|protein pigeon homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSAP, check out the GSAP Infographic
![GSAP infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PION GSAP Antibody (A09313)
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