Anti-PIK3R5/Pi 3 Kinase P101 Antibody

PIK3R5 antibody

Boster Bio Anti-PIK3R5/Pi 3 Kinase P101 Antibody (Catalog # A02827). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A02827
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-PIK3R5/Pi 3 Kinase P101 Antibody

View all PIK3R5 Antibodies

SKU/Catalog Number

A02827

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-PIK3R5/Pi 3 Kinase P101 Antibody (Catalog # A02827). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PIK3R5/Pi 3 Kinase P101 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02827)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human PIK3R5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02827 is reactive to PIK3R5 in Human, Mouse

Reconstitution

Calculated molecular weight

97348 MW

Background of PIK3R5

Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma membrane via interaction with beta-gamma G protein dimers. Required for G protein-mediated activation of PIK3CG By similarity.

The MGC Project Team;Genome Res. 14:2121-2127(2004).
Sjoeblom T.,Science 314:268-274(2006).
Suire S., Curr. Biol. 15:566-570(2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02827 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For PIK3R5 (Source: Uniprot.org, NCBI)

Gene Name

PIK3R5

Full Name

Phosphoinositide 3-kinase regulatory subunit 5

Weight

97348 MW

Alternative Names

Phosphoinositide 3-kinase regulatory subunit 5;PI3-kinase regulatory subunit 5;PI3-kinase p101 subunit;Phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit;PtdIns-3-kinase regulatory subunit;Protein FOAP-2;PtdIns-3-kinase p101;p101-PI3K;PIK3R5; PIK3R5 F730038I15Rik, FOAP-2, P101-PI3K, p101 phosphoinositide-3-kinase regulatory subunit 5 phosphoinositide 3-kinase regulatory subunit 5|PI3-kinase p101 subunit|phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit|protein FOAP-2|ptdIns-3-kinase p101

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PIK3R5, check out the PIK3R5 Infographic

PIK3R5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PIK3R5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-PIK3R5/Pi 3 Kinase P101 Antibody

Question

We are currently using anti-PIK3R5/Pi 3 Kinase P101 antibody A02827 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

The anti-PIK3R5/Pi 3 Kinase P101 antibody (A02827) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-26

Question

Would anti-PIK3R5/Pi 3 Kinase P101 antibody A02827 work on zebrafish IHC with peripheral blood leukocyte spleen?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

Our lab technicians have not validated anti-PIK3R5/Pi 3 Kinase P101 antibody A02827 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PIK3R5/Pi 3 Kinase P101 antibody A02827 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish peripheral blood leukocyte spleen in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-26

Question

I see that the anti-PIK3R5/Pi 3 Kinase P101 antibody A02827 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-14

Question

Is a blocking peptide available for product anti-PIK3R5/Pi 3 Kinase P101 antibody (A02827)?

Verified Customer

Verified customer

Asked: 2018-03-14

Answer

We do provide the blocking peptide for product anti-PIK3R5/Pi 3 Kinase P101 antibody (A02827). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-03-14

Order DetailsPrice
A02827-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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