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Product Info Summary
| SKU: | A06707-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-PIK3R3 Antibody (C-term)
View all PI 3-Kinase p55 gamma Antibodies
SKU/Catalog Number
A06707-1
Size
80 µl
Description
Boster Bio Anti-PIK3R3 Antibody (C-term) (Catalog # A06707-1). Tested in IF, WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PIK3R3 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06707-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PIK3R3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-346 amino acids from the C-terminal region of human PIK3R3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06707-1 is reactive to PIK3R3 in Human
Applications
A06707-1 is guaranteed for IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54448 Da
Background of PI 3-Kinase p55 gamma
PIK3R3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:100
WB: 1:1000
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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Fluorescent image of U251 cells stained with PIK3R3 Antibody (C-term). PIK3R3 Antibody (C-term) was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of lysate from MDA-MB-453 cell line, using PI3KR3 Antibody (G331) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIK3R3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Protein Target Info & Infographic
Gene/Protein Information For PIK3R3 (Source: Uniprot.org, NCBI)
Gene Name
PIK3R3
Full Name
Phosphatidylinositol 3-kinase regulatory subunit gamma
Weight
54448 Da
Superfamily
PI3K p85 subunit family
Alternative Names
100% homology to SWISS-PROT Q92569; DKFZp686P05226; FLJ41892; p55; p55PIK; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphoinositide-3-kinase, regulatory subunit 3 (gamma); phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma); phosphoinositide-3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); PI 3Kinase p55 gamma; PI 3-Kinase p55 gamma; PI3K regulatory subunit gamma; PI3-kinase subunit p55-gamma; PIK3R3; regulatory subunit, polypeptide 3 (p55, gamma) PIK3R3 p55, p55-GAMMA, p55PIK phosphoinositide-3-kinase regulatory subunit 3 phosphatidylinositol 3-kinase regulatory subunit gamma|PI3-kinase regulatory subunit gamma|PI3-kinase subunit p55-gamma|PI3K regulatory subunit gamma|phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma|phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma)|phosphoinositide-3-kinase, regulatory subunit 3 (gamma)|ptdIns-3-kinase regulatory subunit p55-gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIK3R3, check out the PIK3R3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIK3R3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIK3R3 Antibody (C-term) (A06707-1)
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