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Product Info Summary
| SKU: | PA1680 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband®
View all PI 3-Kinase p85 beta Antibodies
SKU/Catalog Number
PA1680
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody catalog # PA1680. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1680)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PIK3R2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1680 is reactive to PIK3R2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
85 kDa
Calculated molecular weight
81545 MW
Background of PI 3-Kinase p85 beta
PIK3R2 (Phosphatidylinositol 3-kinase, regulatory subunit 2), also called p85-Beta, is an enzyme that in humans is encoded by the PIK3R2 gene. The PIK3R2 gene is mapped on 19p13.11. Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1680 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Testis tissue, 293T whole cell, HELA whole cell, mouse liver tissue, mouse brain tissue
IHC: human mammary cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Testis tissue lysates,
Lane 2: 293T whole cell lysates,
Lane 3: HELA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody (Catalog # PA1680) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85KD. The expected band size for PIK3R2 is at 85KD.
Click image to see more details
Figure 2. IHC analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680).
PIK3R2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PIK3R2 Antibody (PA1680) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. Western blot analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody (Catalog # PA1680) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85KD. The expected band size for PIK3R2 is at 85KD.
Protein Target Info & Infographic
Gene/Protein Information For PIK3R2 (Source: Uniprot.org, NCBI)
Gene Name
PIK3R2
Full Name
Phosphatidylinositol 3-kinase regulatory subunit beta
Weight
81545 MW
Superfamily
PI3K p85 subunit family
Alternative Names
Phosphatidylinositol 3-kinase regulatory subunit beta;PI3-kinase regulatory subunit beta;PI3K regulatory subunit beta;PtdIns-3-kinase regulatory subunit beta;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta;PI3-kinase subunit p85-beta;PtdIns-3-kinase regulatory subunit p85-beta;PIK3R2; PIK3R2 MPPH, MPPH1, P85B, p85, p85-BETA phosphoinositide-3-kinase regulatory subunit 2 phosphatidylinositol 3-kinase regulatory subunit beta|PI3-kinase subunit p85-beta|PI3K regulatory subunit beta|phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta|phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)|phosphoinositide-3-kinase regulatory subunit beta|phosphoinositide-3-kinase, regulatory subunit 2 (beta)|ptdIns-3-kinase regulatory subunit p85-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIK3R2, check out the PIK3R2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIK3R2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband® (PA1680)
Hello CJ!
PA1680 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
PI3K p85 β regulatory subunit deficiency does not affect NK cell differentiation and increases NKG2D‐mediated activation
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Customer Q&As
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5 Customer Q&As for Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband®
Question
Do you have a BSA free version of anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 available?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-06
Question
Has PA1680 been tested with mouse samples?
Verified customer
Asked: 2019-03-15
Answer
We have result image of the Anti-PI 3 Kinase P85 Beta/PIK3R2 Antibody (PA1680) on mouse samples for WB. However, we don't have a qualified IHC image with mouse samples.
Boster Scientific Support
Answered: 2019-03-18
Question
I see that the anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-25
Question
Is this PA1680 anti-PI 3 Kinase p85 beta/PIK3R2 antibody reactive to the isotypes of PIK3R2?
G. Anderson
Verified customer
Asked: 2017-10-24
Answer
The immunogen of PA1680 anti-PI 3 Kinase p85 beta/PIK3R2 antibody is A synthetic peptide corresponding to a sequence in the middle region of human PIK3R2 (447-461aa KVYHQQYQDKSREYD), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-24
Question
Will anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 work on pig WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
Our lab technicians have not tested anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 on pig. You can run a BLAST between pig and the immunogen sequence of anti-PI 3 Kinase p85 beta/PIK3R2 antibody PA1680 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-20
