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Product Info Summary
| SKU: | M00914 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal Antibody
SKU/Catalog Number
M00914
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal Antibody (Catalog # M00914). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00914)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: MACIF/1193
Isotype
IgM, kappa
Immunogen
Recombinant full-length human CD59 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00914 is reactive to CD59 in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
14177 MW
Background of CD59
Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). It is useful for study on GPI-anchored proteins, PNH and CD59 functions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00914 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Tongue stained with Anti-CD59 Monoclonal Antibody (MACIF/1193)
Protein Target Info & Infographic
Gene/Protein Information For CD59 (Source: Uniprot.org, NCBI)
Gene Name
CD59
Full Name
CD59 glycoprotein
Weight
14177 MW
Alternative Names
CD59 glycoprotein;1F5 antigen;20 kDa homologous restriction factor;HRF-20;HRF20;MAC-inhibitory protein;MAC-IP;MEM43 antigen;Membrane attack complex inhibition factor;MACIF;Membrane inhibitor of reactive lysis;MIRL;Protectin;CD59;CD59;MIC11, MIN1, MIN2, MIN3, MSK21; CD59 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20 CD59 molecule (CD59 blood group) CD59 glycoprotein|1F5 |20 kDa homologous restriction factor|CD59 p18-20 ( by antibodies 16.3A5, EJ16, EJ30, EL32 and G344)|CD59 blood group |CD59 molecule, complement regulatory protein|Ly-6-like protein|MEM43 |T cell-activating protein|human leukocyte MIC11|lymphocytic CD59/MEM43|membrane attack complex (MAC) inhibition factor|membrane attack complex inhibition factor|membrane inhibitor of reactive lysis|protectin|surface anitgen recognized by 16.3A5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD59, check out the CD59 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD59: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal Antibody (M00914)
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No publications found for M00914
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Customer Q&As
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16 Customer Q&As for Anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal Antibody
Question
Is this M00914 anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody reactive to the isotypes of CD59?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
The immunogen of M00914 anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody is Recombinant full-length human CD59 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-05
Question
We have been able to see staining in human milk. Are there any suggestions? Is anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody supposed to stain milk positively?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
From what I have seen in literature milk does express CD59. From what I have seen in Uniprot.org, CD59 is expressed in chorionic villus, t-cell, blood, colon, milk, liver, urine, among other tissues. Regarding which tissues have CD59 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1383553
Colon, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Milk, Pubmed ID: 18780401
T-cell, Pubmed ID: 2475570
Urine, Pubmed ID: 7520819
Boster Scientific Support
Answered: 2020-05-01
Question
I was wanting to use to test anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 on human t-cell for research purposes, then I may be interested in using anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The products we sell, including anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-10
Question
Here is the WB image, lot number and protocol we used for t-cell using anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-04
Question
Will anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 work on zebrafish IF with colon?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
Our lab technicians have not tested anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish colon in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-01
Question
We were well pleased with the WB result of your anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody. However we have seen positive staining in colon cell membrane using this antibody. Is that expected? Could you tell me where is CD59 supposed to be expressed?
J. Dhar
Verified customer
Asked: 2019-04-05
Answer
From what I have seen in literature, colon does express CD59. Generally CD59 expresses in cell membrane. Regarding which tissues have CD59 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1383553
Colon, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Milk, Pubmed ID: 18780401
T-cell, Pubmed ID: 2475570
Urine, Pubmed ID: 7520819
Boster Scientific Support
Answered: 2019-04-05
Question
Is a blocking peptide available for product anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody (M00914)?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
We do provide the blocking peptide for product anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody (M00914). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-06
Question
Is there a BSA free version of anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 available?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-09
Question
We have tried in the past anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody for IF on blood in a previous experiment. I am using human, and We are going to use the antibody for IHC next. I am interested in examining blood as well as milk in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
I have checked the website and datasheets of our anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody and it appears that M00914 has been tested on human in both IF and IHC. Thus M00914 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-26
Question
Does anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 work for IF with t-cell?
Verified Customer
Verified customer
Asked: 2017-08-29
Answer
According to the expression profile of t-cell, CD59 is highly expressed in t-cell. So, it is likely that anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 will work for IF with t-cell.
Boster Scientific Support
Answered: 2017-08-29
Question
I have a question about product M00914, anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Kulkarni
Verified customer
Asked: 2017-08-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00914 anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-18
Question
We are currently using anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
The anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody (M00914) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-08
Question
Does M00914 anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Jones
Verified customer
Asked: 2014-07-03
Answer
You can see on the product datasheet, M00914 anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-07-03
Question
I was wanting to use your anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody for IF for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?
J. Brown
Verified customer
Asked: 2014-01-15
Answer
It shows on the product datasheet, M00914 anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody has been validated for IF, IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-15
Question
I see that the anti-CD59 / Complement Regulatory Protein / Protectin Monoclonal antibody M00914 works with IF, what is the protocol used to produce the result images on the product page?
A. Jones
Verified customer
Asked: 2013-06-25
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-06-25
