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Product Info Summary
| SKU: | A01707 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband®
SKU/Catalog Number
A01707
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody catalog # A01707. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01707)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PIAS1 recombinant protein (Position: Q96-K300).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01707 is reactive to PIAS1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
71.836kDa
Background of PIAS1
E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01707 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HepG2 whole cell, human Jurkat whole cell, human HEK293 whole cell, human Raji whole cell, human K562 whole cell, human Hela whole cell
ICC/IF: A431 cell
FCM: PC-3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PIAS1 using anti-PIAS1 antibody (A01707).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS1 antigen affinity purified polyclonal antibody (Catalog # A01707) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS1 at approximately 72KD. The expected band size for PIAS1 is at 72KD.
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Figure 2. IF analysis of PIAS1 using anti-PIAS1 antibody (A01707).
PIAS1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PIAS1 Antibody (A01707) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-PIAS1 antibody (A01707).
Overlay histogram showing PC-3 cells stained with A01707 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIAS1 Antibody (A01707, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PIAS1 (Source: Uniprot.org, NCBI)
Gene Name
PIAS1
Full Name
E3 SUMO-protein ligase PIAS1
Weight
71.836kDa
Superfamily
PIAS family
Alternative Names
E3 SUMO-protein ligase PIAS1; DEAD/H box-binding protein 1; E3 SUMO-protein transferase PIAS1; Gu-binding protein; GBP; Protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein; PIAS1; DDXBP1 PIAS1 DDXBP1, GBP, GU/RH-II, ZMIZ3 protein inhibitor of activated STAT 1 E3 SUMO-protein ligase PIAS1|AR interacting protein|DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1|DEAD/H box-binding protein 1|E3 SUMO-protein transferase PIAS1|RNA helicase II-binding protein|gu-binding protein|protein inhibitor of activated STAT protein 1|zinc finger, MIZ-type containing 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIAS1, check out the PIAS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIAS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband® (A01707)
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