Anti-PIAS1+PIAS2+PIAS3 Rabbit Monoclonal Antibody

Figure 1. Western blot analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS1+PIAS2+PIAS3 antigen affinity purified polyclonal antibody (Catalog # M01707) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS1+PIAS2+PIAS3 at approximately 76 kDa. The expected band size for PIAS1+PIAS2+PIAS3 is at 76 kDa.

Figure 2. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 3. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 4. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 5. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 6. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 7. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 8. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 9. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 10. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of non-small cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 11. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of non-small cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 12. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 13. IHC analysis of PIAS1+PIAS2+PIAS3 using anti-PIAS1+PIAS2+PIAS3 antibody (M01707).
PIAS1+PIAS2+PIAS3 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIAS1+PIAS2+PIAS3 Antibody (M01707) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.