Product Info Summary
SKU: | A01398T319 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-MEF-2 (T319) MEF2A Antibody
SKU/Catalog Number
A01398T319
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-MEF-2 (T319) MEF2A Antibody catalog # A01398T319. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-MEF-2 (T319) MEF2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01398T319)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MEF-2 around the phosphorylation site of T319.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01398T319 is reactive to MEF2A in Human, Mouse, Rat
Applications
A01398T319 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54811 MW
Background of MEF2A
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
Abujiang Pataer, et,al. (2002) Cancer Res ; 62: 2239.
K. D. Ryman, et,al. (2005) J. Virol ; 79: 1487 - 1499.
Susana Guerra, et,al. (2006) J. Biol. Chem ; 281: 18734 - 18745.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
![a01398t319 anti phospho mef 2 t319 mef2a antibody wb testing 1 a01398t319 anti phospho mef 2 t319 mef2a antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01398t319-anti-phospho-mef-2-t319-mef2a-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-Phospho-MEF-2 (T319) MEF2A Antibody A01398T319
Western Blot (WB) analysis of VEC cells using Phospho-MEF-2 (T319) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
![a01398t319 anti phospho mef 2 t319 mef2a antibody wb testing 2 a01398t319 anti phospho mef 2 t319 mef2a antibody wb testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01398t319-anti-phospho-mef-2-t319-mef2a-antibody-wb-testing-2.jpg)
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Figure 2. Western blotting validation for Anti-Phospho-MEF-2 (T319) MEF2A Antibody A01398T319
Western Blot (WB) analysis of specific cells using Phospho-MEF-2 (T319) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MEF2A (Source: Uniprot.org, NCBI)
Gene Name
MEF2A
Full Name
Myocyte-specific enhancer factor 2A
Weight
54811 MW
Superfamily
MEF2 family
Alternative Names
ADCAD1; MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancerfactor 2A); MEF2; myocyte enhancer factor 2A; myocyte-specific enhancer factor 2A; RSRFC4; RSRFC9; Serum response factor-like protein 1 MEF2A ADCAD1, RSRFC4, RSRFC9, mef2 myocyte enhancer factor 2A myocyte-specific enhancer factor 2A|MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A)|serum response factor-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MEF2A, check out the MEF2A Infographic
![MEF2A infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MEF2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-MEF-2 (T319) MEF2A Antibody (A01398T319)
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