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Product Info Summary
| SKU: | A00754Y319 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-ZAP-70 (Y319) Antibody
SKU/Catalog Number
A00754Y319
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-ZAP-70 (Y319) Antibody catalog # A00754Y319. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-ZAP-70 (Y319) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00754Y319)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr319. AA range:286-335
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00754Y319 is reactive to ZAP70 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
69872 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00754Y319 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ZAP-70 (Phospho-Tyr319) antibody
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Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr319) antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary antibody was diluted at 1:200 (4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200
Protein Target Info & Infographic
Gene/Protein Information For ZAP70 (Source: Uniprot.org, NCBI)
Gene Name
ZAP70
Full Name
Tyrosine-protein kinase ZAP-70
Weight
69872 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase ZAP-70;2.7.10.2;70 kDa zeta-chain associated protein;Syk-related tyrosine kinase;ZAP70;SRK; ZAP70 ADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70 zeta chain of T cell receptor associated protein kinase 70 tyrosine-protein kinase ZAP-70|70 kDa zeta-associated protein|70 kDa zeta-chain associated protein|syk-related tyrosine kinase|zeta chain of T cell receptor associated protein kinase 70kDa|zeta-chain (TCR) associated protein kinase 70kDa|zeta-chain associated protein kinase, 70kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZAP70, check out the ZAP70 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZAP70: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-ZAP-70 (Y319) Antibody (A00754Y319)
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