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Product Info Summary
| SKU: | P02474 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-TRF1 (Ser219) TERF1 Antibody
SKU/Catalog Number
P02474
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-TRF1 (Ser219) TERF1 Antibody (Catalog # P02474). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-TRF1 (Ser219) TERF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02474)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from human TRF1 around the phosphorylation site of S219.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P02474 is reactive to TERF1 in Human, Mouse
Reconstitution
Calculated molecular weight
50246 MW
Background of TRF-1
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
Tao WA, et al. (2005) Nat Methods. 2(8): 591-598.
Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135.
Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P02474 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1/500 - 1/2000
ELISA: 1/5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of 293 cells using Phospho-TRF1 (S219) Polyclonal antibody
Protein Target Info & Infographic
Gene/Protein Information For TERF1 (Source: Uniprot.org, NCBI)
Gene Name
TERF1
Full Name
Telomeric repeat-binding factor 1
Weight
50246 MW
Alternative Names
Telomeric repeat-binding factor 1;NIMA-interacting protein 2;TTAGGG repeat-binding factor 1;Telomeric protein Pin2/TRF1;TERF1;PIN2, TRBF1, TRF, TRF1; TERF1 PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1 telomeric repeat binding factor 1 telomeric repeat-binding factor 1|NIMA-interacting protein 2|TTAGGG repeat-binding factor 1|telomeric protein Pin2/TRF1|telomeric repeat binding factor (NIMA-interacting) 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TERF1, check out the TERF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TERF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-TRF1 (Ser219) TERF1 Antibody (P02474)
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No publications found for P02474
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Customer Q&As
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6 Customer Q&As for Anti-Phospho-TRF1 (Ser219) TERF1 Antibody
Question
Will anti-Phospho-TRF1 (Ser219) Polyclonal antibody P02474 work on primate WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
Our lab technicians have not tested anti-Phospho-TRF1 (Ser219) Polyclonal antibody P02474 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Phospho-TRF1 (Ser219) Polyclonal antibody P02474 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-17
Question
We are currently using anti-Phospho-TRF1 (Ser219) Polyclonal antibody P02474 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The anti-Phospho-TRF1 (Ser219) Polyclonal antibody (P02474) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-25
Question
We want to test anti-Phospho-TRF1 (Ser219) Polyclonal antibody P02474 on human cervix carcinoma for research purposes, then I may be interested in using anti-Phospho-TRF1 (Ser219) Polyclonal antibody P02474 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The products we sell, including anti-Phospho-TRF1 (Ser219) Polyclonal antibody P02474, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-18
Question
I see that the anti-Phospho-TRF1 (Ser219) Polyclonal antibody P02474 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-21
Question
I have a question about product P02474, anti-Phospho-TRF1 (Ser219) Polyclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Yang
Verified customer
Asked: 2015-09-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P02474 anti-Phospho-TRF1 (Ser219) Polyclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-09-18
Question
Do you have a BSA free version of anti-Phospho-TRF1 (Ser219) Polyclonal antibody P02474 available?
K. Rodriguez
Verified customer
Asked: 2014-08-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-TRF1 (Ser219) Polyclonal antibody P02474 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-08-21
