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Product Info Summary
| SKU: | P01423-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody
SKU/Catalog Number
P01423-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody catalog # P01423-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01423-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Smad5 (S463/465)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01423-1 is reactive to SMAD5 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
130 kDa
Calculated molecular weight
52258 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01423-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human pancreas tissue
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Smad5 in (1)Mouse brain tissue lysate; (2)Rat brain tissue lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For SMAD5 (Source: Uniprot.org, NCBI)
Gene Name
SMAD5
Full Name
Mothers against decapentaplegic homolog 5
Weight
52258 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5;SMAD5;MADH5; SMAD5 DWFC, JV5-1, MADH5 SMAD family member 5 mothers against decapentaplegic homolog 5|MAD, mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD, mothers against DPP homolog 5|mothers against decapentaplegic, drosophila, homolog of, 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD5, check out the SMAD5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody (P01423-1)
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