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Product Info Summary
| SKU: | P01532 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-Ron (Ser1394) Polyclonal Antibody
SKU/Catalog Number
P01532
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Ron (Ser1394) Polyclonal Antibody (Catalog # P01532). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Ron (Ser1394) Polyclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01532)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Ron around the phosphorylation site of S1394.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01532 is reactive to MST1R in Human, Mouse
Reconstitution
Calculated molecular weight
152271 MW
Background of MSPR/Ron
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 - 1854.
Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253.
Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 - 22123.
Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 - C119.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01532 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1/500 - 1/2000
ELISA: 1/40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of 3T3 cells using Phospho-Ron (S1394) Polyclonal antibody
Protein Target Info & Infographic
Gene/Protein Information For MST1R (Source: Uniprot.org, NCBI)
Gene Name
MST1R
Full Name
Macrophage-stimulating protein receptor
Weight
152271 MW
Superfamily
protein kinase superfamily
Alternative Names
Macrophage-stimulating protein receptor;MSP receptor;2.7.10.1;CDw136;Protein-tyrosine kinase 8;p185-Ron;CD136;Macrophage-stimulating protein receptor alpha chain;Macrophage-stimulating protein receptor beta chain;MST1R;PTK8, RON; MST1R CD136, CDw136, NPCA3, PTK8, RON, SEA macrophage stimulating 1 receptor macrophage-stimulating protein receptor|MSP receptor|PTK8 protein tyrosine kinase 8|S13 avian erythroblastosis oncogene homolog|S13 erythroblastosis oncogene homolog|c-met-related tyrosine kinase|p185-Ron
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MST1R, check out the MST1R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MST1R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Ron (Ser1394) Polyclonal Antibody (P01532)
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No publications found for P01532
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-Ron (Ser1394) Polyclonal Antibody
Question
Do you require any kind of licensing agreement with the researcher who wants to evaluate a Boster polyclonal antibody for possible inclusion in a commercialize product?
Verified customer
Asked: 2022-07-05
Answer
Yes, we need to have a licensing agreement, for many purposes but primarily to ensure the continuous supply of this product, which the researcher would need. We should ask them to discuss more details including the expected demand of this antibody. Since this is a polyclonal antibody we need to produce ahead of time and validate to ensure it keeps working properly for the customer.
Boster Scientific Support
Answered: 2022-07-05
Question
I was wanting to use your anti-Phospho-Ron (Ser1394) Polyclonal antibody for WB for human keratinocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human keratinocyte identification?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
It shows on the product datasheet, P01532 anti-Phospho-Ron (Ser1394) Polyclonal antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human keratinocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-16
Question
Is this P01532 anti-Phospho-Ron (Ser1394) Polyclonal antibody reactive to the isotypes of MST1R?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The immunogen of P01532 anti-Phospho-Ron (Ser1394) Polyclonal antibody is Synthesized peptide derived from human Ron around the phosphorylation site of S1394. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-28
Question
I see that the anti-Phospho-Ron (Ser1394) Polyclonal antibody P01532 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-16
Question
We are currently using anti-Phospho-Ron (Ser1394) Polyclonal antibody P01532 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
E. Miller
Verified customer
Asked: 2015-12-10
Answer
The anti-Phospho-Ron (Ser1394) Polyclonal antibody (P01532) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-10
