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Product Info Summary
| SKU: | PA1817 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Hyaluronidase-2 HYAL2 Antibody Picoband®
SKU/Catalog Number
PA1817
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hyaluronidase-2 HYAL2 Antibody catalog # PA1817. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hyaluronidase-2 HYAL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1817)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HYAL2, different from the related mouse and rat sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1817 is reactive to HYAL2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
54 kDa
Calculated molecular weight
53860 MW
Background of HYAL2
HYAL2 (hyaluronoglucosaminidase 2) also known as hyaluronidase 2, LuCa-2, is an enzyme that in humans is encoded by the HYAL2 gene. HYAL2 cDNAs encode a preprotein with an N-terminal signal peptide. Northern blot analysis indicated that HYAL2 was expressed in all human tissues tested except adult brain, and Western blot analysis detected Hyal2protein in all mouse tissues examined except adult brain. The HYAL2 gene is mapped on 3p21.31. HYAL2 is a glycosylphosphatidylinositol (GPI)-anchored protein on the cell surface and serves as a receptor for entry into the cell of the jaagsiekte sheep retrovirus (JSRV). The findings of Rai et al. (2001) that HYAL2 is a GPI-anchored protein on the cell surface showed that GFP would likely be cleaved from HYAL2 during GPI addition, leaving HYAL2 on the cell surface and resulting in GFP transit to the lysosome for degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1817 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: HELA Whole Cell, SMMC Whole Cell, COLO320 Whole Cell, MCF-7 Whole Cell, HT1080 Whole Cell
Validation Images & Assay Conditions
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Anti-HYAL2 antibody, PA1817, Western blotting
All lanes: Anti HYAL2 (PA1817) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 54KD
Observed bind size: 54KD
Protein Target Info & Infographic
Gene/Protein Information For HYAL2 (Source: Uniprot.org, NCBI)
Gene Name
HYAL2
Full Name
Hyaluronidase-2
Weight
53860 MW
Superfamily
glycosyl hydrolase 56 family
Alternative Names
Hyaluronidase-2;Hyal-2;3.2.1.35;Hyaluronoglucosaminidase-2;Lung carcinoma protein 2;LuCa-2;HYAL2;LUCA2; HYAL2 LUCA2 hyaluronidase 2 hyaluronidase-2|PH-20 homolog|PH20 homolog|hyal-2|hyaluronoglucosaminidase 2|lung carcinoma protein 2|lysosomal hyaluronidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HYAL2, check out the HYAL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HYAL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hyaluronidase-2 HYAL2 Antibody Picoband® (PA1817)
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No publications found for PA1817
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Customer Q&As
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7 Customer Q&As for Anti-Hyaluronidase-2 HYAL2 Antibody Picoband®
Question
I have a question about product PA1817, anti-HYAL2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1817 anti-HYAL2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-06
Question
Will anti-HYAL2 antibody PA1817 work on monkey WB with kidney?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
Our lab technicians have not validated anti-HYAL2 antibody PA1817 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-HYAL2 antibody PA1817 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-18
Question
Does PA1817 anti-HYAL2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
It shows on the product datasheet, PA1817 anti-HYAL2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-16
Question
Our lab want to know about to test anti-HYAL2 antibody PA1817 on mouse placenta synovium for research purposes, then I may be interested in using anti-HYAL2 antibody PA1817 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The products we sell, including anti-HYAL2 antibody PA1817, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-17
Question
We are currently using anti-HYAL2 antibody PA1817 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
The anti-HYAL2 antibody (PA1817) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-12
Question
Is this PA1817 anti-HYAL2 antibody reactive to the isotypes of HYAL2?
V. Moore
Verified customer
Asked: 2017-05-16
Answer
The immunogen of PA1817 anti-HYAL2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human HYAL2(142-157aa VRNWQDKDVYRRLSRQ), different from the related mouse and rat sequences by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-16
Question
Will anti-HYAL2 antibody PA1817 work for WB with placenta synovium?
N. Jha
Verified customer
Asked: 2014-06-04
Answer
According to the expression profile of placenta synovium, HYAL2 is highly expressed in placenta synovium. So, it is likely that anti-HYAL2 antibody PA1817 will work for WB with placenta synovium.
Boster Scientific Support
Answered: 2014-06-04
