Product Info Summary
SKU: | A00075S446 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-Raf-B (S446) BRAF Antibody
SKU/Catalog Number
A00075S446
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Raf-B (S446) BRAF Antibody catalog # A00075S446. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Raf-B (S446) BRAF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00075S446)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Raf-B around the phosphorylation site of S446.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00075S446 is reactive to BRAF in Human, Mouse
Applications
A00075S446 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
84437 MW
Background of B-Raf
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. ATP-Binding Cassette Sub-Family B Member 1 (ABCB1, also named P-glycoprotein) is a plasma membrane-associated multidrug transporter that utilizes the energy of ATP hydrolysis to pump toxic xenobiotics out of cells. Unique features of ABCB1 are its very broad substrate specificity and its basal ATPase activity in the absence of transport substrates. Human ABCB1 plays an important role in absorption, distribution, metabolism, excretion and toxicity of pharmacologically relevant drugs. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anti-cancer drugs. This protein also functions as a transporter across the blood-brain barrier.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
![a00075s446 anti phospho raf b s446 braf antibody ihc testing 3 a00075s446 anti phospho raf b s446 braf antibody ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a00075s446-anti-phospho-raf-b-s446-braf-antibody-ihc-testing-3.jpg)
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Figure 3. Immunohistochemistry validation of BRAF using Anti-Phospho-Raf-B (S446) BRAF Antibody (A00075S446).
Immunohistochemical analysis of paraffin-embedded Human brain using Anti-Phospho-Raf-B (S446) polyclonal antibody. Antibody was diluted at 1:100 (4°C![a00075s446 anti phospho raf b s446 braf antibody wb testing 1 a00075s446 anti phospho raf b s446 braf antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a00075s446-anti-phospho-raf-b-s446-braf-antibody-wb-testing-1.jpg)
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Figure 1. Western blotting validation for Anti-Phospho-Raf-B (S446) BRAF Antibody A00075S446
Western Blot (WB) analysis of K562 and 293T lysates using Anti-Phospho-Raf-B (S446) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
![a00075s446 anti phospho raf b s446 braf antibody wb testing 2 a00075s446 anti phospho raf b s446 braf antibody wb testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a00075s446-anti-phospho-raf-b-s446-braf-antibody-wb-testing-2.jpg)
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Figure 2. Western blotting validation for Anti-Phospho-Raf-B (S446) BRAF Antibody A00075S446
Western Blot (WB) analysis of 293 cells using Anti-Phospho-Raf-B (S446) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For BRAF (Source: Uniprot.org, NCBI)
Gene Name
BRAF
Full Name
Serine/threonine-protein kinase B-raf
Weight
84437 MW
Superfamily
protein kinase superfamily
Alternative Names
94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRaf; B-Raf; BRAF1; B-RAF1; BRAF1MGC126806; EC 2.7.11.1; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B1FLJ95109 BRAF B-RAF1, B-raf1, NS7, RAFB1, BRAF B-Raf proto-oncogene, serine/threonine kinase serine/threonine-protein kinase B-raf|94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|B-Raf serine/threonine-protein|murine sarcoma viral (v-raf) oncogene homolog B1|proto-oncogene B-Raf|v-raf murine sarcoma viral oncogene homolog B|v-raf murine sarcoma viral oncogene homolog B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRAF, check out the BRAF Infographic
![BRAF infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRAF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Raf-B (S446) BRAF Antibody (A00075S446)
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