Click image to see more details
Product Info Summary
| SKU: | P00300-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-p62 (Ser28) SQSTM1 Antibody
View all p62/SQSTM1 Antibodies
SKU/Catalog Number
P00300-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-p62 (Ser28) SQSTM1 Antibody (Catalog # P00300-1). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-p62 (Ser28) SQSTM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00300-1)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser28 of human p62, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00300-1 is reactive to SQSTM1 in Human
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
47687 MW
Background of p62/SQSTM1
p62, also known as sequestosome1 (SQSTM1), is a shuttle protein transporting polyubiquitinated proteins for both proteasomal and lysosomal degradation. p62 is an integral component of inclusions in brains of various neurodegenerative disorders, including Alzheimer disease (AD) neurofibrillary tangles (NFTs) and Lewy bodies in Parkinson disease (Nogalaska et al., 2009). p62 plays an important role in the protection of cells from the toxicity of misfolded proteins by enhancing aggregate formation especially in the later stages (Nakaso et al., 2004). Phosphorylation of Ser-28 has recently been demonstrated to be related to the pathogenesis of Parkinson’s disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00300-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of Jurkat cell lysate showing specific immunolabeling of the ~48 kDa p62 phosphorylated at Ser28 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For SQSTM1 (Source: Uniprot.org, NCBI)
Gene Name
SQSTM1
Full Name
Sequestosome-1
Weight
47687 MW
Alternative Names
A170 antibody, DMRV antibody, EBI 3 associated protein of 60 kDa antibody, EBI 3 associated protein p60 antibody, EBI3 associated protein of 60 kDa antibody, EBI3 associated protein p60 antibody, EBI3-associated protein of 60 kDa antibody, EBIAP antibody, FTDALS3 antibody, MGC127197 antibody, ORCA antibody, OSF-6 antibody, Osi antibody, OSIL antibody, Oxidative stress induced like antibody, p60 antibody, p62 antibody, p62B antibody, Paget disease of bone 3 antibody, PDB 3 antibody, PDB3 antibody, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody, Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody, PKC-zeta-interacting protein antibody, Protein kinase C-zeta-interacting protein antibody, Sequestosome 1 antibody, Sequestosome-1 antibody, SQSTM 1 antibody, SQSTM_HUMAN antibody, Sqstm1 antibody, STAP antibody, STONE14 antibody, Ubiquitin binding protein p62 antibody, Ubiquitin-binding protein p62 antibody, ZIP 3 antibody, ZIP antibody, ZIP3 antibody SQSTM1 A170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B sequestosome 1 sequestosome-1|EBI3-associated protein of 60 kDa|EBI3-associated protein p60|EBIAP|autophagy receptor p62|oxidative stress induced like|phosphotyrosine independent ligand for the Lck SH2 domain p62|phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa|ubiquitin-binding protein p62
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SQSTM1, check out the SQSTM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SQSTM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-p62 (Ser28) SQSTM1 Antibody (P00300-1)
Hello CJ!
P00300-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Song L,Yao L,Zhang L,Piao Z,Lu Y.Schizandrol A protects against Aβ1-42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons.Naunyn Schmiedebergs Arch Pharmacol.2020 Sep;393(9):1739-1752.doi:10.1007/s00
Species: Human
P00300-1 usage in article: APP:WB, SAMPLE:SH-SY5Y CELLS, DILUTION:1:400
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Phospho-p62 (Ser28) SQSTM1 Antibody
Question
Would anti-Phospho-p62 (Ser28) antibody P00300-1 work on monkey WB with placenta?
K. Jackson
Verified customer
Asked: 2020-03-16
Answer
Our lab technicians have not tested anti-Phospho-p62 (Ser28) antibody P00300-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Phospho-p62 (Ser28) antibody P00300-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-16
Question
I see that the anti-Phospho-p62 (Ser28) antibody P00300-1 works with WB, what is the protocol used to produce the result images on the product page?
B. Jones
Verified customer
Asked: 2020-01-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-15
Question
I would like to test anti-Phospho-p62 (Ser28) antibody P00300-1 on human skin uterus for research purposes, then I may be interested in using anti-Phospho-p62 (Ser28) antibody P00300-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The products we sell, including anti-Phospho-p62 (Ser28) antibody P00300-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-18
Question
Do you have a BSA free version of anti-Phospho-p62 (Ser28) antibody P00300-1 available?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-p62 (Ser28) antibody P00300-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-22
Question
My team were happy with the WB result of your anti-Phospho-p62 (Ser28) antibody. However we have observed positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is SQSTM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
From what I have seen in literature, liver does express SQSTM1. Generally SQSTM1 expresses in cytoplasm, cytosol. Regarding which tissues have SQSTM1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8551575
Caudate nucleus, and Trachea, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 8650207, 16964243, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Pancreas, Placenta, Skin, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-02
Question
We are currently using anti-Phospho-p62 (Ser28) antibody P00300-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
The anti-Phospho-p62 (Ser28) antibody (P00300-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-14
Question
I was wanting to use your anti-Phospho-p62 (Ser28) antibody for WB for human skin uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skin uterus identification?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
As indicated on the product datasheet, P00300-1 anti-Phospho-p62 (Ser28) antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-23
Question
Is this P00300-1 anti-Phospho-p62 (Ser28) antibody reactive to the isotypes of SQSTM1?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
The immunogen of P00300-1 anti-Phospho-p62 (Ser28) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser28 of p62. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin uterus using anti-Phospho-p62 (Ser28) antibody P00300-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-17
Question
My question regards using your anti-Phospho-p62 (Ser28) antibody for nrif signals cell death from the nucleus studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
We appreciate your inquiry. This P00300-1 anti-Phospho-p62 (Ser28) antibody is tested on jurkat cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-01
Question
Will P00300-1 anti-Phospho-p62 (Ser28) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
You can see on the product datasheet, P00300-1 anti-Phospho-p62 (Ser28) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-27
Question
We have been able to see staining in human b-cell. Do you have any suggestions? Is anti-Phospho-p62 (Ser28) antibody supposed to stain b-cell positively?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
From literature b-cell does express SQSTM1. From Uniprot.org, SQSTM1 is expressed in left adrenal gland cortex, b-cell, cervix carcinoma, caudate nucleus trachea, pancreas, placenta, skin uterus, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SQSTM1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8551575
Caudate nucleus, and Trachea, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 8650207, 16964243, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Pancreas, Placenta, Skin, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-03-13
Question
Is a blocking peptide available for product anti-Phospho-p62 (Ser28) antibody (P00300-1)?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
We do provide the blocking peptide for product anti-Phospho-p62 (Ser28) antibody (P00300-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-12
Question
Here is the WB image, lot number and protocol we used for skin uterus using anti-Phospho-p62 (Ser28) antibody P00300-1. Please let me know if you require anything else.
K. Wu
Verified customer
Asked: 2017-05-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-09
Question
Can you help my question with product P00300-1, anti-Phospho-p62 (Ser28) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Miller
Verified customer
Asked: 2016-11-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00300-1 anti-Phospho-p62 (Ser28) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-11-15
Question
Will anti-Phospho-p62 (Ser28) antibody P00300-1 work for WB with skin uterus?
N. Williams
Verified customer
Asked: 2015-04-14
Answer
According to the expression profile of skin uterus, SQSTM1 is highly expressed in skin uterus. So, it is likely that anti-Phospho-p62 (Ser28) antibody P00300-1 will work for WB with skin uterus.
Boster Scientific Support
Answered: 2015-04-14
