Product Info Summary
SKU: | P00450 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Nucleophosmin (T199) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00450
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-Nucleophosmin (T199) Rabbit Monoclonal Antibody catalog # P00450. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Nucleophosmin (T199) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00450)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
28N18
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-Nucleophosmin (T199)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00450 is reactive to NPM1 in Human
Reconstitution
Observed Molecular Weight
38-40 kDa
Calculated molecular weight
73052 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00450 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Phospho-Nucleophosmin (T199) expression in (1) HeLa cell lysate; (2) HeLa cell treated with CA lysate.
Protein Target Info & Infographic
Gene/Protein Information For NPM1 (Source: Uniprot.org, NCBI)
Gene Name
NPM1
Full Name
Nucleophosmin
Weight
73052 MW
Superfamily
nucleoplasmin family
Alternative Names
RAF proto-oncogene serine/threonine-protein kinase;2.7.11.1;Proto-oncogene c-RAF;cRaf;Raf-1;RAF1;RAF; NPM1 B23, NPM nucleophosmin 1 nucleophosmin|nucleolar protein NO38|nucleophosmin (nucleolar phosphoprotein B23, numatrin)|nucleophosmin/nucleoplasmin family, member 1|testicular tissue protein Li 128
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPM1, check out the NPM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Nucleophosmin (T199) Rabbit Monoclonal Antibody (P00450)
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No publications found for P00450
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