Product Info Summary
SKU: | P00284 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-Phospho-NF-Kappa B p65 (S529) RELA Rabbit Monoclonal Antibody
SKU/Catalog Number
P00284
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-NF-Kappa B p65 (S529) RELA Rabbit Monoclonal Antibody catalog # P00284. Tested in WB, Flow Cytometry, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-NF-Kappa B p65 (S529) RELA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00284)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AbN84
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NF-Kappa B p65
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00284 is reactive to RELA in Human
Applications
P00284 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Observed Molecular Weight
62 kDa
Calculated molecular weight
60219 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:50
IP 1:50
Validation Images & Assay Conditions
![p00284 wb p00284 wb](https://www.bosterbio.com/media/catalog/product/p/0/p00284-wb.jpg)
Click image to see more details
Western blot analysis of Phospho-NF-κB p65 (S529) expression in HeLa cell lysates treated with Calyculin A and TNF-alpha.
Protein Target Info & Infographic
Gene/Protein Information For RELA (Source: Uniprot.org, NCBI)
Gene Name
RELA
Full Name
Transcription factor p65
Weight
60219 MW
Alternative Names
NF kB p65; NFkB p65; NF-kB p65; p65; rela p65; RelA; MGC131774; nf kb p65; NFkB p65; NF-kB p65; NFKB3; NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65; p65 flow cytometry; p65 immunohistochemistry; p65; p65RelA; rela p65; RelA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight po RELA CMCU, NFKB3, p65 RELA proto-oncogene, NF-kB subunit transcription factor p65|NF-kappa-B p65delta3|NF-kappa-B transcription factor p65|nuclear factor NF-kappa-B p65 subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RELA, check out the RELA Infographic
![RELA infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RELA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-NF-Kappa B p65 (S529) RELA Rabbit Monoclonal Antibody (P00284)
Hello CJ!
P00284 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tibatan MA,Önay Uçar E.Long non-coding RNA NKILA regulates expression of HSP90α, NF-κB and β-catenin proteins in the MCF-7 breast cancer cell line.Mol Biol Rep.2021 Jun 14.doi:10.1007/s11033-021-06482-y.Epub ahead of print.PMID:34125329.
Species: Human
P00284 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:1:1000
Guo L,Jiang B,Li D,Xiao X. Nephroprotective Effect of Adropinin Against Streptozotocin-Induced Diabetic Nephropathy in Rats: Inflammatory Mechanism and YAP/TAZ Factor.Drug Des Devel Ther.2021 Feb 16;15:589-600.doi:10. 2147/DDDT.S294009.PMID:33623368;PMCID
Species: Rat
P00284 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
P00284 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:1000
Down-regulation of HMGB1 expression by shRNA constructs inhibits the bioactivity of urothelial carcinoma cell lines via the NF-?B pathway
microRNA-146a inhibits cancer metastasis by downregulating VEGF through dual pathways in hepatocellular carcinoma
MiR199b Suppresses Expression of Hypoxia-Inducible Factor 1? (HIF-1?) in Prostate Cancer Cells
Oligomannurarate sulfate blocks tumor growth by inhibiting NF-?B activation
Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells
Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-?B activation
Fenofibrate, a PPAR? agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB
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