Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody

Lat antibody

Boster Bio Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody catalog # P01654. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: P01654
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

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Product Name

Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody

View all Lat Antibodies

SKU/Catalog Number

P01654

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody catalog # P01654. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01654)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOH-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-LAT (Y191)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P01654 is reactive to LAT in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

156 kDa

Calculated molecular weight

27930 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

P01654 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30

Positive Control

WB: Jurkat cell

Validation Images & Assay Conditions

Gene/Protein Information For LAT (Source: Uniprot.org, NCBI)

Gene Name

LAT

Full Name

Linker for activation of T-cells family member 1

Weight

27930 MW

Alternative Names

Linker for activation of T-cells family member 1;36 kDa phospho-tyrosine adapter protein;pp36;p36-38;LAT; Lat|p36-38, pp36|linker for activation of T cells|linker for activation of T-cells family member 1|36 kDa phospho-tyrosine adapter protein|linker protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LAT, check out the LAT Infographic

LAT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

P01654 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The characteristics of herpes simplex virus type 1 infection in rhesus macaques and the associated pathological features

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5 Customer Q&As for Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody

Question

Our lab were well pleased with the WB result of your anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody. However we have observed positive staining in platelet cell membrane using this antibody. Is that expected? Could you tell me where is LAT supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

From what I have seen in literature, platelet does express LAT. Generally LAT expresses in cell membrane. Regarding which tissues have LAT expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 15616553
Colon, Pubmed ID: 15489334
Leukemia, Pubmed ID: 9489702
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Thymus, Pubmed ID: 9529333

Boster Scientific Support

Answered: 2020-04-21

Question

We have been able to see staining in mouse leukocyte. Any tips? Is anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody supposed to stain leukocyte positively?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

According to literature leukocyte does express LAT. According to Uniprot.org, LAT is expressed in leukocyte, leukemia, thymus, bone marrow, colon, leukemic t-cell, platelet, liver, among other tissues. Regarding which tissues have LAT expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 15616553
Colon, Pubmed ID: 15489334
Leukemia, Pubmed ID: 9489702
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Thymus, Pubmed ID: 9529333

Boster Scientific Support

Answered: 2019-07-01

Question

I am interested in using your anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody for intracellular signal transduction studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

Thanks for your inquiry. This P01654 anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-13

Question

We are currently using anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody P01654 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2017-08-10

Answer

The anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody (P01654) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-08-10

Question

We ordered your anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody for IF on liver a few months ago. I am using mouse, and We intend to use the antibody for ICC next. We need examining liver as well as leukemia in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?

T. Wu

Verified customer

Asked: 2016-08-26

Answer

I looked at the website and datasheets of our anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody and I see that P01654 has been validated on mouse in both IF and ICC. Thus P01654 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-08-26

Order DetailsPrice
P01654

100μL

$370
P01654-30ul

30μL sample

$99
P01654-carrier-free-1ml

Carrier Free

$2590

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