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Product Info Summary
| SKU: | P01654 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody
SKU/Catalog Number
P01654
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody catalog # P01654. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01654)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOH-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-LAT (Y191)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01654 is reactive to LAT in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
156 kDa
Calculated molecular weight
27930 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01654 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-LAT (Y220) in Jurkat cell treated with CD3 lysate.
Protein Target Info & Infographic
Gene/Protein Information For LAT (Source: Uniprot.org, NCBI)
Gene Name
LAT
Full Name
Linker for activation of T-cells family member 1
Weight
27930 MW
Alternative Names
Linker for activation of T-cells family member 1;36 kDa phospho-tyrosine adapter protein;pp36;p36-38;LAT; Lat|p36-38, pp36|linker for activation of T cells|linker for activation of T-cells family member 1|36 kDa phospho-tyrosine adapter protein|linker protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LAT, check out the LAT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody (P01654)
Hello CJ!
P01654 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The characteristics of herpes simplex virus type 1 infection in rhesus macaques and the associated pathological features
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-LAT (Y191) Rabbit Monoclonal Antibody
Question
Our lab were well pleased with the WB result of your anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody. However we have observed positive staining in platelet cell membrane using this antibody. Is that expected? Could you tell me where is LAT supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
From what I have seen in literature, platelet does express LAT. Generally LAT expresses in cell membrane. Regarding which tissues have LAT expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 15616553
Colon, Pubmed ID: 15489334
Leukemia, Pubmed ID: 9489702
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Thymus, Pubmed ID: 9529333
Boster Scientific Support
Answered: 2020-04-21
Question
We have been able to see staining in mouse leukocyte. Any tips? Is anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
According to literature leukocyte does express LAT. According to Uniprot.org, LAT is expressed in leukocyte, leukemia, thymus, bone marrow, colon, leukemic t-cell, platelet, liver, among other tissues. Regarding which tissues have LAT expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 15616553
Colon, Pubmed ID: 15489334
Leukemia, Pubmed ID: 9489702
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Thymus, Pubmed ID: 9529333
Boster Scientific Support
Answered: 2019-07-01
Question
I am interested in using your anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody for intracellular signal transduction studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Thanks for your inquiry. This P01654 anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-13
Question
We are currently using anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody P01654 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-10
Answer
The anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody (P01654) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-10
Question
We ordered your anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody for IF on liver a few months ago. I am using mouse, and We intend to use the antibody for ICC next. We need examining liver as well as leukemia in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
T. Wu
Verified customer
Asked: 2016-08-26
Answer
I looked at the website and datasheets of our anti-Phospho-LAT (Y191) Rabbit Monoclonal antibody and I see that P01654 has been validated on mouse in both IF and ICC. Thus P01654 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-08-26
