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Product Info Summary
| SKU: | A00268S636 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-IRS-1 (S636) Antibody
SKU/Catalog Number
A00268S636
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-IRS-1 (S636) Antibody catalog # A00268S636. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-IRS-1 (S636) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00268S636)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser636. AA range:603-652
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00268S636 is reactive to IRS1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
131591 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00268S636 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of SH-SY5Y cells using Phospho-IRS-1 (S636) polyclonal antibody diluted at 1:2000
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Western Blot analysis of RAW using Phospho-IRS-1 (S636) polyclonal antibody diluted at 1:2000
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Western blot analysis of 293T 3T3 lysis using Phospho-IRS-1 (S636) antibody. antibody was diluted at 1:2000
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Immunohistochemical analysis of paraffin-embedded Human brain. antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser636) antibody
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Immunofluorescence analysis of MCF7 cells, using IRS-1 (Phospho-Ser636) antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser636) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells and COLO205 cells, using IRS-1 (Phospho-Ser636) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For IRS1 (Source: Uniprot.org, NCBI)
Gene Name
IRS1
Full Name
Insulin receptor substrate 1
Weight
131591 MW
Alternative Names
Insulin receptor substrate 1;IRS-1;IRS1; IRS1 HIRS-1 insulin receptor substrate 1 insulin receptor substrate 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRS1, check out the IRS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-IRS-1 (S636) Antibody (A00268S636)
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