Click image to see more details
Product Info Summary
| SKU: | P06762 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-HSL (Ser855) Polyclonal Antibody
View all Hormone-sensitive Lipase/HSL Antibodies
SKU/Catalog Number
P06762
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-HSL (Ser855) Polyclonal Antibody (Catalog # P06762). Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-HSL (Ser855) Polyclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P06762)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from human HSL around the phosphorylation site of S855.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P06762 is reactive to LIPE in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
116598 MW
Background of Hormone-sensitive Lipase/HSL
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P06762 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
ELISA: 1/20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of L929 using p-HSL (S855) antibody.
Protein Target Info & Infographic
Gene/Protein Information For LIPE (Source: Uniprot.org, NCBI)
Gene Name
LIPE
Full Name
Hormone-sensitive lipase
Weight
116598 MW
Superfamily
GDXG' lipolytic enzyme family
Alternative Names
Hormone-sensitive lipase;HSL;3.1.1.79;LIPE; LIPE AOMS4, FPLD6, HSL, LHS, REH lipase E, hormone sensitive type hormone-sensitive lipase|lipase, hormone-sensitive|monoacylglycerol lipase LIPE|retinyl ester hydrolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIPE, check out the LIPE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIPE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-HSL (Ser855) Polyclonal Antibody (P06762)
Hello CJ!
No publications found for P06762
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-HSL (Ser855) Polyclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Phospho-HSL (Ser855) Polyclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Phospho-HSL (Ser855) Polyclonal Antibody
Question
Do you require any kind of licensing agreement with the researcher who wants to evaluate a Boster polyclonal antibody for possible inclusion in a commercialize product?
Verified customer
Asked: 2022-07-05
Answer
Yes, we need to have a licensing agreement, for many purposes but primarily to ensure the continuous supply of this product, which the researcher would need. We should ask them to discuss more details including the expected demand of this antibody. Since this is a polyclonal antibody we need to produce ahead of time and validate to ensure it keeps working properly for the customer.
Boster Scientific Support
Answered: 2022-07-05
Question
Will anti-Phospho-HSL (Ser855) Polyclonal antibody P06762 work on goat WB with testis?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Our lab technicians have not tested anti-Phospho-HSL (Ser855) Polyclonal antibody P06762 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Phospho-HSL (Ser855) Polyclonal antibody P06762 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-17
Question
Would P06762 anti-Phospho-HSL (Ser855) Polyclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
You can see on the product datasheet, P06762 anti-Phospho-HSL (Ser855) Polyclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-28
Question
I am interested in to test anti-Phospho-HSL (Ser855) Polyclonal antibody P06762 on mouse subcutaneous adipose tissue for research purposes, then I may be interested in using anti-Phospho-HSL (Ser855) Polyclonal antibody P06762 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The products we sell, including anti-Phospho-HSL (Ser855) Polyclonal antibody P06762, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-11
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for subcutaneous adipose tissue using anti-Phospho-HSL (Ser855) Polyclonal antibody P06762. Let me know if you need anything else.
A. Moore
Verified customer
Asked: 2015-10-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-23
