Product Info Summary
SKU: | A02726S260 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-hnRNP C1/2 (S260) Antibody
View all hnRNP C1 + C2 Antibodies
SKU/Catalog Number
A02726S260
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-hnRNP C1/2 (S260) Antibody catalog # A02726S260. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-hnRNP C1/2 (S260) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02726S260)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human hnRNP C1/2 around the phosphorylation site of S260.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02726S260 is reactive to HNRNPC in Human, Mouse, Rat
Applications
A02726S260 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
33670 MW
Background of hnRNP C1 + C2
Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.
Burd C.G., Proc. Natl. Acad. Sci. U.S.A. 86:9788-9792(1989).
Swanson M.S., Mol. Cell. Biol. 7:1731-1739(1987).
Merrill B.M., Nucleic Acids Res. 17:8441-8449(1989).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain, using hnRNP C1/2 (Phospho-Ser260) Antibody. The picture on the right is blocked with the phospho peptide.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Click image to see more details
Western blot analysis of 293 cells using Phospho-hnRNP C1/2 (S260) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Protein Target Info & Infographic
Gene/Protein Information For HNRNPC (Source: Uniprot.org, NCBI)
Gene Name
HNRNPC
Full Name
Heterogeneous nuclear ribonucleoproteins C1/C2
Weight
33670 MW
Superfamily
RRM HNRPC family
Alternative Names
C1; C2; heterogeneous nuclear ribonucleoprotein C (C1/C2); heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2; HNRNP; hnRNPC; HNRPC; MGC104306; MGC105117; MGC117353; MGC131677; nuclear ribonucleoprotein particle C1 protein; nuclear ribonucleoprotein particle C2 protein; SNRPC HNRNPC C1, C2, HNRNP, HNRPC, SNRPC heterogeneous nuclear ribonucleoprotein C heterogeneous nuclear ribonucleoproteins C1/C2|heterogeneous nuclear ribonucleoprotein C (C1/C2)|hnRNP C1/C2|nuclear ribonucleoprotein particle C1 protein|nuclear ribonucleoprotein particle C2 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNRNPC, check out the HNRNPC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNRNPC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-hnRNP C1/2 (S260) Antibody (A02726S260)
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