Product Info Summary
SKU: | A03296-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-LMX1B Antibody
SKU/Catalog Number
A03296-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-LMX1B Antibody (Catalog # A03296-1). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LMX1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03296-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human LMX1B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03296-1 is reactive to LMX1B in Human, Mouse
Applications
A03296-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44917 MW
Background of LMX1B
Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.
Dreyer S.D., Nat. Genet. 19:47-50(1998).
Vollrath D., Hum. Mol. Genet. 7:1091-1098(1998).
McIntosh I., Am. J. Hum. Genet. 63:1651-1658(1998).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells, 293 cells and HUVEC cells, using LMX1B antibody A03296-1.
Protein Target Info & Infographic
Gene/Protein Information For LMX1B (Source: Uniprot.org, NCBI)
Gene Name
LMX1B
Full Name
LIM homeobox transcription factor 1-beta
Weight
44917 MW
Alternative Names
LIM homeobox transcription factor 1, beta; LIM homeobox transcription factor 1-beta; LIM/homeobox protein 1.2; LIM/homeobox protein LMX1B; LMX1.2; LMX-1.2; MGC138325; MGC142051; NPS1 LMX1B FSGS10, LMX1.2, NPS1 LIM homeobox transcription factor 1 beta LIM homeobox transcription factor 1-beta|LIM/homeobox protein 1.2|LMX-1.2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LMX1B, check out the LMX1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LMX1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LMX1B Antibody (A03296-1)
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No publications found for A03296-1
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Customer Q&As
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5 Customer Q&As for Anti-LMX1B Antibody
Question
Do you have a BSA free version of anti-LMX1B antibody A03296-1 available?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-LMX1B antibody A03296-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-13
Question
Would anti-LMX1B antibody A03296-1 work for WB with substantia nigra?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
According to the expression profile of substantia nigra, LMX1B is highly expressed in substantia nigra. So, it is likely that anti-LMX1B antibody A03296-1 will work for WB with substantia nigra.
Boster Scientific Support
Answered: 2019-12-02
Question
We are currently using anti-LMX1B antibody A03296-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The anti-LMX1B antibody (A03296-1) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-06
Question
Would anti-LMX1B antibody A03296-1 work on canine WB with substantia nigra?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Our lab technicians have not validated anti-LMX1B antibody A03296-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-LMX1B antibody A03296-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine substantia nigra in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-06
Question
I am interested in to test anti-LMX1B antibody A03296-1 on human substantia nigra for research purposes, then I may be interested in using anti-LMX1B antibody A03296-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-29
Answer
The products we sell, including anti-LMX1B antibody A03296-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-29