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Product Info Summary
| SKU: | MP12477 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Histone H3 (S10) HIST1H3A Rabbit Monoclonal Antibody
SKU/Catalog Number
MP12477
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H3 (S10) HIST1H3A Rabbit Monoclonal Antibody catalog # MP12477. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H3 (S10) HIST1H3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP12477)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BAE-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Histone H3 (S10).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
MP12477 is reactive to HIST1H3A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
17 kDa
Calculated molecular weight
15 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MP12477 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human HepG2 whole cell, human 293T whole cell, rat RH35 whole cell, mouse NIH/3T3 whole cell
IHC: mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Phospho-Histone H3 (S10) using anti-Phospho-Histone H3 (S10) antibody (MP12477).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-Histone H3 (S10) antigen affinity purified monoclonal antibody (MP12477) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospho-Histone H3 (S10) at approximately 17 kDa. The expected band size for Phospho-Histone H3 (S10) is at 15 kDa.
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Immunohistochemical analysis of paraffin-embedded mouse liver, using Phospho-Histone H3 (S10) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For HIST1H3A (Source: Uniprot.org, NCBI)
Gene Name
HIST1H3A
Full Name
Histone H3.1
Weight
15 kDa
Superfamily
histone H3 family
Alternative Names
Red fluorescent protein eqFP611;GFP-like chromoprotein; H3C1 H3/A0, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, H3C1 H3 clustered histone 1 histone H3.1|H3 histone family, member A|histone 1, H3a|histone H3/a|histone cluster 1 H3 family member a|histone cluster 1, H3a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIST1H3A, check out the HIST1H3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIST1H3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Histone H3 (S10) HIST1H3A Rabbit Monoclonal Antibody (MP12477)
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