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Product Info Summary
| SKU: | A00595S22 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-HDAC6 (S22) Antibody
SKU/Catalog Number
A00595S22
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-HDAC6 (S22) Antibody catalog # A00595S22. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-HDAC6 (S22) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00595S22)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human HDAC6 around the phosphorylation site of Ser22. AA range:7-56
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00595S22 is reactive to HDAC6 in Human, Mouse
Reconstitution
Calculated molecular weight
131419 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00595S22 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Immunofluorescence analysis of Hela cell. 1, HDAC6 (Phospho- Ser22) polyclonal antibody (red) was diluted at 1:200 (4° overnight). Galectin-3 Monoclonal antibody (6G2) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
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Western Blot analysis of 3T3 cells using Phospho-HDAC6 (S22) polyclonal antibody diluted at 1:500
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Immunohistochemical analysis of paraffin-embedded Human brain. antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HDAC6 (Phospho-Ser22) antibody
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Immunofluorescence analysis of HepG2 cells, using HDAC6 (Phospho-Ser22) antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human testis, using HDAC6 (Phospho-Ser22) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from NIH/3T3 cells treated with Anisomycin 25ug/ml 30', using HDAC6 (Phospho-Ser22) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For HDAC6 (Source: Uniprot.org, NCBI)
Gene Name
HDAC6
Full Name
Histone deacetylase 6
Weight
131419 MW
Superfamily
histone deacetylase family
Alternative Names
Histone deacetylase 6;HD6;3.5.1.98;HDAC6;KIAA0901;JM21; HDAC6 CPBHM, HD6, JM21, PPP1R90 histone deacetylase 6 histone deacetylase 6|protein phosphatase 1, regulatory subunit 90|tubulin-lysine deacetylase HDAC6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC6, check out the HDAC6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-HDAC6 (S22) Antibody (A00595S22)
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