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Product Info Summary
| SKU: | P00593 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Phospho-GATA3 (S308) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00593
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-GATA3 (S308) Rabbit Monoclonal Antibody catalog # P00593. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-GATA3 (S308) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00593)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DHF-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-GATA3 (S308)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00593 is reactive to GATA3 in Human
Reconstitution
Observed Molecular Weight
185 kDa
Calculated molecular weight
47916 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00593 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
IP 1:50
Positive Control
WB: Jurkat cell
IHC: human bladder cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Phospho-GATA3 (S308) Jurkat cell lysate treated with Bromo-cAMP.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-GATA3 (S308) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For GATA3 (Source: Uniprot.org, NCBI)
Gene Name
GATA3
Full Name
Trans-acting T-cell-specific transcription factor GATA-3
Weight
47916 MW
Alternative Names
Trans-acting T-cell-specific transcription factor GATA-3;GATA-binding factor 3;GATA3; GATA3 HDR, HDRS GATA binding protein 3 trans-acting T-cell-specific transcription factor GATA-3|GATA-binding factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GATA3, check out the GATA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GATA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-GATA3 (S308) Rabbit Monoclonal Antibody (P00593)
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No publications found for P00593
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Phospho-GATA3 (S308) Rabbit Monoclonal Antibody
Question
I was wanting to use using your anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody for chromatin remodeling studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Thanks for your inquiry. This P00593 anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IP, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-20
Question
Do you have a BSA free version of anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593 available?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-23
Question
We were well pleased with the WB result of your anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody. However we have been able to see positive staining in endometrium epithelium nucleus. using this antibody. Is that expected? Could you tell me where is GATA3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
From literature, endometrium epithelium does express GATA3. Generally GATA3 expresses in nucleus. Regarding which tissues have GATA3 expression, here are a few articles citing expression in various tissues:
Cervix, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
T-cell, Pubmed ID: 1827068, 2017177, 2050118
Boster Scientific Support
Answered: 2020-02-11
Question
Will P00593 anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
As indicated on the product datasheet, P00593 anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-29
Question
Is this P00593 anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody reactive to the isotypes of GATA3?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The immunogen of P00593 anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody is A synthesized peptide derived from human Phospho-GATA3 (S308). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-10
Question
Is a blocking peptide available for product anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody (P00593)?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We do provide the blocking peptide for product anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody (P00593). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-03
Question
We are currently using anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody (P00593) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-13
Question
We bought anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody for WB on t-cell in the past. I am using human, and We are going to use the antibody for IHC next. I was wanting to use examining t-cell as well as endometrium epithelium in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
I viewed the website and datasheets of our anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody and I see that P00593 has been tested on human in both WB and IHC. Thus P00593 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-22
Question
Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-26
Question
I am looking for to test anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593 on human leukemic t-cell for research purposes, then I may be interested in using anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The products we sell, including anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-24
Question
I have a question about product P00593, anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00593 anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-11
Question
I was wanting to use your anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
It shows on the product datasheet, P00593 anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody has been tested for IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-10
Question
Does anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
According to the expression profile of leukemic t-cell, GATA3 is highly expressed in leukemic t-cell. So, it is likely that anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-07-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-31
Question
We have been able to see staining in human leukemic t-cell. What should we do? Is anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody supposed to stain leukemic t-cell positively?
D. Collins
Verified customer
Asked: 2016-11-30
Answer
From literature leukemic t-cell does express GATA3. From Uniprot.org, GATA3 is expressed in endometrium epithelium, t-cell, cervix placenta, leukemic t-cell, among other tissues. Regarding which tissues have GATA3 expression, here are a few articles citing expression in various tissues:
Cervix, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
T-cell, Pubmed ID: 1827068, 2017177, 2050118
Boster Scientific Support
Answered: 2016-11-30
Question
I see that the anti-Phospho-GATA3 (S308) Rabbit Monoclonal antibody P00593 works with IHC, what is the protocol used to produce the result images on the product page?
P. Krishna
Verified customer
Asked: 2013-05-17
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-17
