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Product Info Summary
| SKU: | PB9199 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GATA3 Antibody Picoband®
SKU/Catalog Number
PB9199
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GATA3 Antibody Picoband® catalog # PB9199. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GATA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9199)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GATA3 recombinant protein (Position: M1-E197). Human GATA3 shares 95% amino acid (aa) sequence identity with mouse GATA3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9199 is reactive to GATA3 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
47916 MW
Background of GATA-3
Trans-acting T-cell-specific transcription factor GATA-3, also named HDR or GATA3, is a protein that in humans is encoded by the GATA3 gene. It is mapped to 10p14. This gene belongs to the GATA family of transcription factors. It regulates luminal epithelial cell differentiation in the mammary gland. The protein contains two GATA-type zinc fingers, it is an important regulator of T cell development and plays an important role in endothelial cell biology. GATA3 has been shown to promote the secretion of IL-4, IL-5, and IL-13 from Th2 cells, and induces the differentiation of Th0 cells towards this T cell subtype while suppressing their differentiation towards Th1 cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9199 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat brain tissue, human MCF-7 whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GATA3 using anti-GATA3 antibody (PB9199).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human GATA3 protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 antigen affinity purified polyclonal antibody (Catalog # PB9199) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 39 kDa. The expected band size for GATA3 is at 39 kDa.
Click image to see more details
Figure 2. Western blot analysis of GATA3 using anti-GATA3 antibody (PB9199).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 antigen affinity purified polyclonal antibody (Catalog # PB9199) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 48 kDa. The expected band size for GATA3 is at 48 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GATA3 (Source: Uniprot.org, NCBI)
Gene Name
GATA3
Full Name
Trans-acting T-cell-specific transcription factor GATA-3
Weight
47916 MW
Alternative Names
Trans-acting T-cell-specific transcription factor GATA-3;GATA-binding factor 3;GATA3; GATA3 HDR, HDRS GATA binding protein 3 trans-acting T-cell-specific transcription factor GATA-3|GATA-binding factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GATA3, check out the GATA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GATA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GATA3 Antibody Picoband® (PB9199)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-GATA3 Antibody Picoband®
Question
My question regards using your anti-GATA3 antibody for chromatin remodeling studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
I appreciate your inquiry. This PB9199 anti-GATA3 antibody is tested on rat brain tissue, tissue lysate, hepg2 whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-08
Question
Our team were happy with the WB result of your anti-GATA3 antibody. However we have been able to see positive staining in cervix placenta nucleus. using this antibody. Is that expected? Could you tell me where is GATA3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
Based on literature, cervix placenta does express GATA3. Generally GATA3 expresses in nucleus. Regarding which tissues have GATA3 expression, here are a few articles citing expression in various tissues:
Cervix, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
T-cell, Pubmed ID: 1827068, 2017177, 2050118
Boster Scientific Support
Answered: 2020-04-29
Question
I have a question about product PB9199, anti-GATA3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9199 anti-GATA3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-09
Question
We have seen staining in human t-cell. What should we do? Is anti-GATA3 antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
Based on literature t-cell does express GATA3. Based on Uniprot.org, GATA3 is expressed in endometrium epithelium, t-cell, cervix placenta, leukemic t-cell, among other tissues. Regarding which tissues have GATA3 expression, here are a few articles citing expression in various tissues:
Cervix, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
T-cell, Pubmed ID: 1827068, 2017177, 2050118
Boster Scientific Support
Answered: 2019-11-22
Question
I see that the anti-GATA3 antibody PB9199 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-08
Question
Is there a BSA free version of anti-GATA3 antibody PB9199 available?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-GATA3 antibody PB9199 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-12
Question
Will anti-GATA3 antibody PB9199 work for WB with t-cell?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
According to the expression profile of t-cell, GATA3 is highly expressed in t-cell. So, it is likely that anti-GATA3 antibody PB9199 will work for WB with t-cell.
Boster Scientific Support
Answered: 2019-05-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-GATA3 antibody PB9199. Let me know if you need anything else.
B. Wu
Verified customer
Asked: 2018-09-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-17
Question
We want to test anti-GATA3 antibody PB9199 on human t-cell for research purposes, then I may be interested in using anti-GATA3 antibody PB9199 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
The products we sell, including anti-GATA3 antibody PB9199, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-24
Question
I was wanting to use your anti-GATA3 antibody for WB for human t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human t-cell identification?
B. Edwards
Verified customer
Asked: 2018-06-07
Answer
As indicated on the product datasheet, PB9199 anti-GATA3 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-07
Question
Is a blocking peptide available for product anti-GATA3 antibody (PB9199)?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
We do provide the blocking peptide for product anti-GATA3 antibody (PB9199). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-19
Question
Is this PB9199 anti-GATA3 antibody reactive to the isotypes of GATA3?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
The immunogen of PB9199 anti-GATA3 antibody is E.coli-derived human GATA3 recombinant protein (Position: M1-E197). Human GATA3 shares 95% amino acid (aa) sequence identity with mouse GATA3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-14
Question
We are currently using anti-GATA3 antibody PB9199 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on bovine tissues as well?
S. Huang
Verified customer
Asked: 2017-10-20
Answer
The anti-GATA3 antibody (PB9199) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-20
Question
Does PB9199 anti-GATA3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
It shows on the product datasheet, PB9199 anti-GATA3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-21
Question
Will anti-GATA3 antibody PB9199 work on canine WB with leukemic t-cell?
H. Mangal
Verified customer
Asked: 2016-10-28
Answer
Our lab technicians have not validated anti-GATA3 antibody PB9199 on canine. You can run a BLAST between canine and the immunogen sequence of anti-GATA3 antibody PB9199 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-10-28
Question
See below the WB image, lot number and protocol we used for t-cell using anti-GATA3 antibody PB9199. Please let me know if you require anything else.
A. Krishna
Verified customer
Asked: 2016-09-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-09-29
