Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269

Product Name

Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269

View all EGFR Antibodies

SKU/Catalog Number

P00023-5

Size

100uL

Form

Liquid

Description

Boster Bio Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 (Catalog # P00023-5). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00023-5)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM269

Isotype

Rabbit IgG

Immunogen

A phospho-peptide corresponding to human Phospho- EGFR (Tyr1173)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to human EGF Receptor only when phosphorylated at Tyr1173. There is no cross-reactivity to EGFR without phosphorylation at Tyr1173.

Reactive Species

P00023-5 is reactive to EGFR in Human

Applications

P00023-5 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

134277 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:5000 dilution.

Validation Images & Assay Conditions

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Figure 1. Western Blotting result
Western Blot of A431 cell nontreated (-) or treated (+) with EGF, using Anti-Phospho-EGFR (Tyr1173) Rabbit Monoclonal Antibody (clone RM269) at a 1:500 dilution, showed Phospho-EGFR (Tyr1173) in EGF-treated A431 cells.

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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-EGFR (Tyr1173) Rabbit Monoclonal Antibody (clone RM269) at a 1:5000 dilution.

Gene/Protein Information For EGFR (Source: Uniprot.org, NCBI)

Alternative Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1

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