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Product Info Summary
| SKU: | P00023-5 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269
SKU/Catalog Number
P00023-5
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 (Catalog # P00023-5). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00023-5)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM269
Isotype
Rabbit IgG
Immunogen
A phospho-peptide corresponding to human Phospho- EGFR (Tyr1173)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human EGF Receptor only when phosphorylated at Tyr1173. There is no cross-reactivity to EGFR without phosphorylation at Tyr1173.
Reactive Species
P00023-5 is reactive to EGFR in Human
Reconstitution
Calculated molecular weight
134277 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00023-5 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:5000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of A431 cell nontreated (-) or treated (+) with EGF, using Anti-Phospho-EGFR (Tyr1173) Rabbit Monoclonal Antibody (clone RM269) at a 1:500 dilution, showed Phospho-EGFR (Tyr1173) in EGF-treated A431 cells.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-EGFR (Tyr1173) Rabbit Monoclonal Antibody (clone RM269) at a 1:5000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For EGFR (Source: Uniprot.org, NCBI)
Gene Name
EGFR
Full Name
Epidermal growth factor receptor
Weight
134277 MW
Superfamily
protein kinase superfamily
Alternative Names
Epidermal growth factor receptor;2.7.10.1;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1;EGFR;ERBB, ERBB1, HER1; EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EGFR, check out the EGFR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EGFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 (P00023-5)
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