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Product Info Summary
| SKU: | A01696T290-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Cot (T290) MAP3K8 Antibody
View all MAP3K8/Tpl2/COT Antibodies
SKU/Catalog Number
A01696T290-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Cot (T290) MAP3K8 Antibody catalog # A01696T290-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Cot (T290) MAP3K8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01696T290-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human COT around the phosphorylation site of Thr290. AA range:256-305
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01696T290-1 is reactive to MAP3K8 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
52925 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01696T290-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-Cot (T290) polyclonal antibody
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using COT (Phospho-Thr290) antibody
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Immunofluorescence analysis of HUVEC cells, using COT (Phospho-Thr290) antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells treated with UV 15', using COT (Phospho-Thr290) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For MAP3K8 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K8
Full Name
Mitogen-activated protein kinase kinase kinase 8
Weight
52925 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase kinase kinase 8;2.7.11.25;Cancer Osaka thyroid oncogene;Proto-oncogene c-Cot;Serine/threonine-protein kinase cot;Tumor progression locus 2;TPL-2;MAP3K8;COT, ESTF; MAP3K8 AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT mitogen-activated protein kinase kinase kinase 8 mitogen-activated protein kinase kinase kinase 8|Ewing sarcoma transformant|augmented in rheumatoid arthritis 2|cot (cancer Osaka thyroid) oncogene|proto-oncogene c-Cot|proto-oncogene serine/threoine protein kinase|tumor progression locus 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K8, check out the MAP3K8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Cot (T290) MAP3K8 Antibody (A01696T290-1)
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