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Product Info Summary
| SKU: | A02631S257-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Caspase-6 (S257) CASP6 Antibody
SKU/Catalog Number
A02631S257-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Caspase-6 (S257) CASP6 Antibody catalog # A02631S257-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Caspase-6 (S257) CASP6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02631S257-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Caspase 6 around the phosphorylation site of Ser257. AA range:223-272
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02631S257-1 is reactive to CASP6 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
33310 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02631S257-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of 293T cells using Phospho-Caspase-6 (S257) polyclonal antibody diluted at 1:1000
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of HUVEC cells, using Caspase 6 (Phospho-Ser257) antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Caspase 6 (Phospho-Ser257) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Caspase 6 (Phospho-Ser257) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For CASP6 (Source: Uniprot.org, NCBI)
Gene Name
CASP6
Full Name
Caspase-6
Weight
33310 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-6;CASP-6;3.4.22.59;Apoptotic protease Mch-2;Caspase-6 subunit p18;Caspase-6 subunit p11;CASP6;MCH2; CASP6 MCH2 caspase 6 caspase-6|apoptotic protease MCH-2|caspase 6, apoptosis-related cysteine peptidase|caspase 6, apoptosis-related cysteine protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP6, check out the CASP6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Caspase-6 (S257) CASP6 Antibody (A02631S257-1)
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No publications found for A02631S257-1
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