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Product Info Summary
| SKU: | P00245-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-BTK (Y223) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00245-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-BTK (Y223) Rabbit Monoclonal Antibody catalog # P00245-1. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-BTK (Y223) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00245-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ECD-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-BTK (Y223)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00245-1 is reactive to BTK in Human
Reconstitution
Observed Molecular Weight
100-110 kDa
Calculated molecular weight
76281 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00245-1 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IP 1:20
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-BTK (Y223) expression in Raji cell lysate treated with pervanadate.
Protein Target Info & Infographic
Gene/Protein Information For BTK (Source: Uniprot.org, NCBI)
Gene Name
BTK
Full Name
Tyrosine-protein kinase BTK
Weight
76281 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase BTK;2.7.10.2;Agammaglobulinemia tyrosine kinase;ATK;B-cell progenitor kinase;BPK;Bruton tyrosine kinase;BTK;AGMX1, ATK, BPK; BTK AGMX1, AT, ATK, BPK, IGHD3, IMD1, PSCTK1, XLA Bruton tyrosine kinase tyrosine-protein kinase BTK|B-cell progenitor kinase|Bruton agammaglobulinemia tyrosine kinase|Brutons tyrosine kinase|agammaglobulinaemia tyrosine kinase|dominant-negative kinase-deficient Brutons tyrosine kinase|truncated Bruton agammaglobulinemia tyrosine kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BTK, check out the BTK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BTK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-BTK (Y223) Rabbit Monoclonal Antibody (P00245-1)
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No publications found for P00245-1
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-BTK (Y223) Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-BTK (Y223) Rabbit Monoclonal antibody P00245-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-Phospho-BTK (Y223) Rabbit Monoclonal antibody (P00245-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
