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Product Info Summary
| SKU: | P04124 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-4.1R (Tyr660) EPB41 Antibody
SKU/Catalog Number
P04124
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-4.1R (Tyr660) EPB41 Antibody (Catalog # P04124). Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-4.1R (Tyr660) EPB41 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04124)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from human 4.1R around the phosphorylation site of Y660.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P04124 is reactive to EPB41 in Human, Mouse
Reconstitution
Calculated molecular weight
97017 MW
Background of EPB41
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P04124 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
ELISA: 1/5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of Jurkat cells using Phospho-4.1R (Y660) Polyclonal antibody
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Western Blot analysis of JK cells using Phospho-4.1R (Y660) Polyclonal antibody
Protein Target Info & Infographic
Gene/Protein Information For EPB41 (Source: Uniprot.org, NCBI)
Gene Name
EPB41
Full Name
Protein 4.1
Weight
97017 MW
Alternative Names
Protein 4.1;P4.1;4.1R;Band 4.1;EPB4.1;EPB41;E41P; EPB41 4.1R, EL1, HE erythrocyte membrane protein band 4.1 protein 4.1|EPB4.1|P4.1|band 4.1|elliptocytosis 1, RH-linked|erythrocyte surface protein band 4.1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPB41, check out the EPB41 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPB41: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-4.1R (Tyr660) EPB41 Antibody (P04124)
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No publications found for P04124
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Phospho-4.1R (Tyr660) EPB41 Antibody
Question
Is this P04124 anti-Phospho-4.1R (Tyr660) Polyclonal antibody reactive to the isotypes of EPB41?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The immunogen of P04124 anti-Phospho-4.1R (Tyr660) Polyclonal antibody is Synthesized peptide derived from human 4.1R around the phosphorylation site of Y660.
Boster Scientific Support
Answered: 2020-05-08
Question
We are currently using anti-Phospho-4.1R (Tyr660) Polyclonal antibody P04124 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The anti-Phospho-4.1R (Tyr660) Polyclonal antibody (P04124) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Phospho-4.1R (Tyr660) Polyclonal antibody P04124. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-22
Question
See attached the WB image, lot number and protocol we used for brain using anti-Phospho-4.1R (Tyr660) Polyclonal antibody P04124. Please let me know if you require anything else.
D. Carter
Verified customer
Asked: 2018-02-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-09
Question
My question regarding product P04124, anti-Phospho-4.1R (Tyr660) Polyclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P04124 anti-Phospho-4.1R (Tyr660) Polyclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-15
Question
Do you have a BSA free version of anti-Phospho-4.1R (Tyr660) Polyclonal antibody P04124 available?
D. Thomas
Verified customer
Asked: 2014-05-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-4.1R (Tyr660) Polyclonal antibody P04124 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-05-06
Question
Is a blocking peptide available for product anti-Phospho-4.1R (Tyr660) Polyclonal antibody (P04124)?
T. Anderson
Verified customer
Asked: 2013-02-12
Answer
We do provide the blocking peptide for product anti-Phospho-4.1R (Tyr660) Polyclonal antibody (P04124). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-02-12
