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Product Info Summary
| SKU: | A05823 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PHLA1 PHLDA1 Antibody
SKU/Catalog Number
A05823
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PHLA1 PHLDA1 Antibody (Catalog # A05823). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHLA1 PHLDA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05823)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PHLA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05823 is reactive to PHLDA1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
45016 MW
Background of Phlda1
Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.
Kuske M.D., Cytogenet. Cell Genet. 89:1-1(2000).
Neef R., Cancer Res. 62:5920-5929(2002).
Hinz T., Cell. Signal. 13:345-352(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05823 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from RAW264.7 cells, using PHLA1 antibody A05823.
Protein Target Info & Infographic
Gene/Protein Information For PHLDA1 (Source: Uniprot.org, NCBI)
Gene Name
PHLDA1
Full Name
Pleckstrin homology-like domain family A member 1
Weight
45016 MW
Alternative Names
Pleckstrin homology-like domain family A member 1;Apoptosis-associated nuclear protein;Proline- and glutamine-rich protein;PQ-rich protein;PQR protein;Proline- and histidine-rich protein;T-cell death-associated gene 51 protein;PHLDA1;PHRIP, TDAG51; Phlda1|DT1P1B1, DT1P1B11, TDAG5, TDAG51, Tda, Tdag|pleckstrin homology like domain, family A, member 1|pleckstrin homology-like domain family A member 1|PQ-rich protein|PQR protein|T-cell death associated|T-cell death-associated gene 51 protein|proline- and glutamine-rich protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHLDA1, check out the PHLDA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHLDA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHLA1 PHLDA1 Antibody (A05823)
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No publications found for A05823
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Customer Q&As
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5 Customer Q&As for Anti-PHLA1 PHLDA1 Antibody
Question
We are currently using anti-PHLA1 antibody A05823 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The anti-PHLA1 antibody (A05823) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-12
Question
Is there a BSA free version of anti-PHLA1 antibody A05823 available?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PHLA1 antibody A05823 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-06
Question
Would A05823 anti-PHLA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
You can see on the product datasheet, A05823 anti-PHLA1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-20
Question
I see that the anti-PHLA1 antibody A05823 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-15
Question
I have a question about product A05823, anti-PHLA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05823 anti-PHLA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-28
